Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer

© 2014, Advanced Research Journals. All rights reserved. The hydroglycolic extracts from Terminaiia chiebula Retzius, Termnnalia belerca Roxb and Rafflesia kerrii Meijer were investigated for total phenolic content (TPC), cytotoxicity, mutagenicity, antimutagenicity and antityrosinase for safety ass...

Full description

Saved in:
Bibliographic Details
Main Authors: Wijittra Nittayajaiprom, Padchanee Sangthong, Sirirat Chancharunee, Angkana Wipatanawin, Pimphaka Wanasawas, Malyn Chulasiri
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84944454376&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53813
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-53813
record_format dspace
spelling th-cmuir.6653943832-538132018-09-04T10:00:32Z Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer Wijittra Nittayajaiprom Padchanee Sangthong Sirirat Chancharunee Angkana Wipatanawin Pimphaka Wanasawas Malyn Chulasiri Medicine Pharmacology, Toxicology and Pharmaceutics © 2014, Advanced Research Journals. All rights reserved. The hydroglycolic extracts from Terminaiia chiebula Retzius, Termnnalia belerca Roxb and Rafflesia kerrii Meijer were investigated for total phenolic content (TPC), cytotoxicity, mutagenicity, antimutagenicity and antityrosinase for safety assessment as novel botanical-based cosmeceutical ingredients. These plant extracts showed TPC between 4.90 ± 0.02 and 112.40 ± 0.08 mg GAE g-1 of extract when using the Folin-Ciocalteu method. The cytotoxicity study revealed that the 50% cytotoxicity dose (CD50) towards normal mouse fibroblast L929 and mouse melanoma B16F10 cell lines was 5.43 ± 0.18 - 39.39 ± 0.14 mg mL-1 and 4.35 ± 0.33 - 58.23 ± 0.18 mg mL-1, respectively. In genotoxicity investigation, it was found that all extracts were not mutagenic at the concentrations up to 87.34 mg 0.1 mL-1 when tested with Salmonella typhhimuruum strains TA98 and TA100 in the presence and absence of metabolic activation (S9 microsomal fraction). The extracts were further tested for antimutagenic activity against 2-aminoanthracene (2-AA) and 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2) which were used as the tested mutagens. Interestingly, all hydroglycolic extracts exhibited the inhibitory effect on the mutagenicity after being induced by 2AA and AF-2 in S. typhimurium strains TA98 and TA100 in the presence and absence of metabolic activation. All plant extracts were further investigated for tyrosinase inhibitory activity. Results showed that all extracts possessed tyrosinase inhibitory activity with 50% inhibitory concentration values (IC50) of 1.27 ± 0.49 - 39.96 ± 0.21 mg mL-1. Overall studies including their antimutagenicity and antityrosinase activities suggest that the hydroglycolic extracts of these three plants may be used as potential candidates for skin-care cosmeceutical ingredients. 2018-09-04T09:58:41Z 2018-09-04T09:58:41Z 2014-01-01 Journal 09750185 2-s2.0-84944454376 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84944454376&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53813
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Medicine
Pharmacology, Toxicology and Pharmaceutics
Wijittra Nittayajaiprom
Padchanee Sangthong
Sirirat Chancharunee
Angkana Wipatanawin
Pimphaka Wanasawas
Malyn Chulasiri
Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer
description © 2014, Advanced Research Journals. All rights reserved. The hydroglycolic extracts from Terminaiia chiebula Retzius, Termnnalia belerca Roxb and Rafflesia kerrii Meijer were investigated for total phenolic content (TPC), cytotoxicity, mutagenicity, antimutagenicity and antityrosinase for safety assessment as novel botanical-based cosmeceutical ingredients. These plant extracts showed TPC between 4.90 ± 0.02 and 112.40 ± 0.08 mg GAE g-1 of extract when using the Folin-Ciocalteu method. The cytotoxicity study revealed that the 50% cytotoxicity dose (CD50) towards normal mouse fibroblast L929 and mouse melanoma B16F10 cell lines was 5.43 ± 0.18 - 39.39 ± 0.14 mg mL-1 and 4.35 ± 0.33 - 58.23 ± 0.18 mg mL-1, respectively. In genotoxicity investigation, it was found that all extracts were not mutagenic at the concentrations up to 87.34 mg 0.1 mL-1 when tested with Salmonella typhhimuruum strains TA98 and TA100 in the presence and absence of metabolic activation (S9 microsomal fraction). The extracts were further tested for antimutagenic activity against 2-aminoanthracene (2-AA) and 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2) which were used as the tested mutagens. Interestingly, all hydroglycolic extracts exhibited the inhibitory effect on the mutagenicity after being induced by 2AA and AF-2 in S. typhimurium strains TA98 and TA100 in the presence and absence of metabolic activation. All plant extracts were further investigated for tyrosinase inhibitory activity. Results showed that all extracts possessed tyrosinase inhibitory activity with 50% inhibitory concentration values (IC50) of 1.27 ± 0.49 - 39.96 ± 0.21 mg mL-1. Overall studies including their antimutagenicity and antityrosinase activities suggest that the hydroglycolic extracts of these three plants may be used as potential candidates for skin-care cosmeceutical ingredients.
format Journal
author Wijittra Nittayajaiprom
Padchanee Sangthong
Sirirat Chancharunee
Angkana Wipatanawin
Pimphaka Wanasawas
Malyn Chulasiri
author_facet Wijittra Nittayajaiprom
Padchanee Sangthong
Sirirat Chancharunee
Angkana Wipatanawin
Pimphaka Wanasawas
Malyn Chulasiri
author_sort Wijittra Nittayajaiprom
title Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer
title_short Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer
title_full Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer
title_fullStr Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer
title_full_unstemmed Mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/Rimeijer
title_sort mutagenicity, antimutagenicity and tyrosinase inhibition activity of hydroglycol extracts from terminalia chiebula retzius, terminalia belerica roxb and rafflesia ke/rimeijer
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84944454376&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53813
_version_ 1681424204838207488