Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers

Relationships of Swiss-type heterocellular HPFH as functions of XmnI-Gγ and HBBP1:rs2071348 polymorphisms and HbF, HbE, MCV and MCH in HbE carriers were evaluated in 52 non-anemic and α-thalassemia-free Thai HbE carriers. HbF and HbE levels were measured using cation-exchange HPLC. MCV and MCH were...

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Main Authors: Sasiwan Kerdpoo, Ektong Limweeraprajak, Thanusak Tatu
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/53825
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spelling th-cmuir.6653943832-538252018-09-04T09:58:53Z Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers Sasiwan Kerdpoo Ektong Limweeraprajak Thanusak Tatu Medicine Relationships of Swiss-type heterocellular HPFH as functions of XmnI-Gγ and HBBP1:rs2071348 polymorphisms and HbF, HbE, MCV and MCH in HbE carriers were evaluated in 52 non-anemic and α-thalassemia-free Thai HbE carriers. HbF and HbE levels were measured using cation-exchange HPLC. MCV and MCH were determined using an automated blood counter. The XmnI-Gγ polymorphism was identified by XmnI digestion of amplified products, and the HBBP1:rs2071348 polymorphism by tetra-ARMS-PCR. HbF levels in HbE carriers were higher than those in normal individuals. HbF levels >0.8 % indicated the Swiss-type heterocellular HPFH in these subjects, rendering a prevalence of 40.4 %. The XmnI-Gγ (+) and HBBP1:rs2071348 (C) alleles were modestly positively correlated with elevated HbF, elevated MCH and lowered HbE values. This study thus confirms the influence of the XmnI-Gγ and HBBP1:rs2071348 polymorphisms on HbF production. The present study demonstrates the association of XmnI-Gγ and HBBP1:rs2071348 with HbF, HbE, MCV and MCH in HbE carriers for the first time, and highlights the effect of elevated HbF production on HbE levels. © 2014 The Japanese Society of Hematology. 2018-09-04T09:58:53Z 2018-09-04T09:58:53Z 2014-01-01 Journal 18653774 09255710 2-s2.0-84899428147 10.1007/s12185-014-1516-z https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899428147&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53825
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Sasiwan Kerdpoo
Ektong Limweeraprajak
Thanusak Tatu
Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers
description Relationships of Swiss-type heterocellular HPFH as functions of XmnI-Gγ and HBBP1:rs2071348 polymorphisms and HbF, HbE, MCV and MCH in HbE carriers were evaluated in 52 non-anemic and α-thalassemia-free Thai HbE carriers. HbF and HbE levels were measured using cation-exchange HPLC. MCV and MCH were determined using an automated blood counter. The XmnI-Gγ polymorphism was identified by XmnI digestion of amplified products, and the HBBP1:rs2071348 polymorphism by tetra-ARMS-PCR. HbF levels in HbE carriers were higher than those in normal individuals. HbF levels >0.8 % indicated the Swiss-type heterocellular HPFH in these subjects, rendering a prevalence of 40.4 %. The XmnI-Gγ (+) and HBBP1:rs2071348 (C) alleles were modestly positively correlated with elevated HbF, elevated MCH and lowered HbE values. This study thus confirms the influence of the XmnI-Gγ and HBBP1:rs2071348 polymorphisms on HbF production. The present study demonstrates the association of XmnI-Gγ and HBBP1:rs2071348 with HbF, HbE, MCV and MCH in HbE carriers for the first time, and highlights the effect of elevated HbF production on HbE levels. © 2014 The Japanese Society of Hematology.
format Journal
author Sasiwan Kerdpoo
Ektong Limweeraprajak
Thanusak Tatu
author_facet Sasiwan Kerdpoo
Ektong Limweeraprajak
Thanusak Tatu
author_sort Sasiwan Kerdpoo
title Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers
title_short Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers
title_full Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers
title_fullStr Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers
title_full_unstemmed Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers
title_sort effect of swiss-type heterocellular hpfh from xmni-<sup>g</sup>γ and hbbp1 polymorphisms on hbf, hbe, mcv and mch levels in thai hbe carriers
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899428147&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53825
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