Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing
© 2014 Institute of Zoology, Chinese Academy of Sciences. This study characterizes the colonization and composition of bacterial flora in dwarf Asian honeybee (Apis florea) larvae and compares bacterial diversity and distribution among different sampling locations. A. florea larvae were collected fr...
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th-cmuir.6653943832-540462018-09-04T10:09:01Z Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing Prakaimuk Saraithong Yihong Li Kanokporn Saenphet Zhou Chen Panuwan Chantawannakul Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © 2014 Institute of Zoology, Chinese Academy of Sciences. This study characterizes the colonization and composition of bacterial flora in dwarf Asian honeybee (Apis florea) larvae and compares bacterial diversity and distribution among different sampling locations. A. florea larvae were collected from 3 locations in Chiang Mai province, Thailand. Bacterial DNA was extracted from each larva using the phenol-chloroform method. Denaturing gradient gel electrophoresis was performed, and the dominant bands were excised from the gels, cloned, and sequenced for bacterial species identification. The result revealed similarities of bacterial community profiles in each individual colony, but differences between colonies from the same and different locations. A. florea larvae harbor bacteria belonging to 2 phyla (Firmicutes and Proteobacteria), 5 classes (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacilli, and Clostridia), 6 genera (Clostridium, Gilliamella, Melissococcus, Lactobacillus, Saccharibacter, and Snodgrassella), and an unknown genus from uncultured bacterial species. The classes with the highest abundance of bacteria were Alphaproteobacteria (34%), Bacilli (25%), Betaproteobacteria (11%), Gammaproteobacteria (10%), and Clostridia (8%), respectively. Similarly, uncultured bacterial species were identified (12%). Environmental bacterial species, such as Saccharibacter floricola, were also found. This is the first study in which sequences closely related to Melissococcus plutonius, the causal pathogen responsible for European foulbrood, have been identified in Thai A. florea larvae. 2018-09-04T10:07:02Z 2018-09-04T10:07:02Z 2015-01-01 Journal 17447917 16729609 2-s2.0-84929188041 10.1111/1744-7917.12155 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929188041&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54046 |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Prakaimuk Saraithong Yihong Li Kanokporn Saenphet Zhou Chen Panuwan Chantawannakul Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing |
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© 2014 Institute of Zoology, Chinese Academy of Sciences. This study characterizes the colonization and composition of bacterial flora in dwarf Asian honeybee (Apis florea) larvae and compares bacterial diversity and distribution among different sampling locations. A. florea larvae were collected from 3 locations in Chiang Mai province, Thailand. Bacterial DNA was extracted from each larva using the phenol-chloroform method. Denaturing gradient gel electrophoresis was performed, and the dominant bands were excised from the gels, cloned, and sequenced for bacterial species identification. The result revealed similarities of bacterial community profiles in each individual colony, but differences between colonies from the same and different locations. A. florea larvae harbor bacteria belonging to 2 phyla (Firmicutes and Proteobacteria), 5 classes (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacilli, and Clostridia), 6 genera (Clostridium, Gilliamella, Melissococcus, Lactobacillus, Saccharibacter, and Snodgrassella), and an unknown genus from uncultured bacterial species. The classes with the highest abundance of bacteria were Alphaproteobacteria (34%), Bacilli (25%), Betaproteobacteria (11%), Gammaproteobacteria (10%), and Clostridia (8%), respectively. Similarly, uncultured bacterial species were identified (12%). Environmental bacterial species, such as Saccharibacter floricola, were also found. This is the first study in which sequences closely related to Melissococcus plutonius, the causal pathogen responsible for European foulbrood, have been identified in Thai A. florea larvae. |
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Journal |
author |
Prakaimuk Saraithong Yihong Li Kanokporn Saenphet Zhou Chen Panuwan Chantawannakul |
author_facet |
Prakaimuk Saraithong Yihong Li Kanokporn Saenphet Zhou Chen Panuwan Chantawannakul |
author_sort |
Prakaimuk Saraithong |
title |
Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing |
title_short |
Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing |
title_full |
Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing |
title_fullStr |
Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing |
title_full_unstemmed |
Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing |
title_sort |
bacterial community structure in apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16s rrna gene sequencing |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929188041&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54046 |
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