Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats

© 2015, Canadian Science Publishing. All rights reserved. Oxidative stress mediated apoptosis of renal tubular cells is a major pathology of gentamicin-induced nephrotoxicity, which is one of the prevailing causes of acute renal failure. Pinocembrin is a major flavonoid found in rhizomes of fingerro...

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Main Authors: Sasivimon Promsan, Krit Jaikumkao, Anchalee Pongchaidecha, Nipon Chattipakorn, Varanuj Chatsudthipong, Phatchawan Arjinajarn, Wilart Pompimon, Anusorn Lungkaphin
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/54097
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-540972018-09-04T10:24:46Z Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats Sasivimon Promsan Krit Jaikumkao Anchalee Pongchaidecha Nipon Chattipakorn Varanuj Chatsudthipong Phatchawan Arjinajarn Wilart Pompimon Anusorn Lungkaphin Biochemistry, Genetics and Molecular Biology Medicine Pharmacology, Toxicology and Pharmaceutics © 2015, Canadian Science Publishing. All rights reserved. Oxidative stress mediated apoptosis of renal tubular cells is a major pathology of gentamicin-induced nephrotoxicity, which is one of the prevailing causes of acute renal failure. Pinocembrin is a major flavonoid found in rhizomes of fingerroot (Boesenbergia pandurata). It has pharmacological and biological activities including antimicrobial, anti-inflammatory, and antioxidant effects. Preclinical studies have suggested that pinocembrin protects rat brain and heart against oxidation and apoptosis induced by ischemia–reperfusion. The aim of the current study was to investigate the mechanisms of renoprotection elicited by pinocembrin in gentamicin-induced nephrotoxicity. Nephrotoxicity was induced in rats by intraperitoneal injection (i.p.) of gentamicin, and pinocembrin was administered via i.p. 30 min before gentamicin treatment for 10 days. Gentamicin-induced nephrotoxicity was indicated by the reduced renal function and renal Oat3 function and expression. Gentamicin treatment also stimulated Nrf2, HO-1, and NQO1, as well as the pro-apoptotic proteins Bax and caspase-3, concomitant with the attenuation of Bcl-XL expression in the renal cortical tissues. Pinocembrin pretreatment improved renal function and renal Oat3 function and reduced oxidative stress and apoptotic conditions. These findings indicate that pinocembrin has a protective effect against gentamicin-induced nephrotoxicity, which may be due in part to its antioxidant and anti-apoptotic effects, subsequently leading to improved renal function. 2018-09-04T10:07:41Z 2018-09-04T10:07:41Z 2015-12-16 Journal 12057541 00084212 2-s2.0-84979966329 10.1139/cjpp-2015-0468 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84979966329&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54097
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Sasivimon Promsan
Krit Jaikumkao
Anchalee Pongchaidecha
Nipon Chattipakorn
Varanuj Chatsudthipong
Phatchawan Arjinajarn
Wilart Pompimon
Anusorn Lungkaphin
Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats
description © 2015, Canadian Science Publishing. All rights reserved. Oxidative stress mediated apoptosis of renal tubular cells is a major pathology of gentamicin-induced nephrotoxicity, which is one of the prevailing causes of acute renal failure. Pinocembrin is a major flavonoid found in rhizomes of fingerroot (Boesenbergia pandurata). It has pharmacological and biological activities including antimicrobial, anti-inflammatory, and antioxidant effects. Preclinical studies have suggested that pinocembrin protects rat brain and heart against oxidation and apoptosis induced by ischemia–reperfusion. The aim of the current study was to investigate the mechanisms of renoprotection elicited by pinocembrin in gentamicin-induced nephrotoxicity. Nephrotoxicity was induced in rats by intraperitoneal injection (i.p.) of gentamicin, and pinocembrin was administered via i.p. 30 min before gentamicin treatment for 10 days. Gentamicin-induced nephrotoxicity was indicated by the reduced renal function and renal Oat3 function and expression. Gentamicin treatment also stimulated Nrf2, HO-1, and NQO1, as well as the pro-apoptotic proteins Bax and caspase-3, concomitant with the attenuation of Bcl-XL expression in the renal cortical tissues. Pinocembrin pretreatment improved renal function and renal Oat3 function and reduced oxidative stress and apoptotic conditions. These findings indicate that pinocembrin has a protective effect against gentamicin-induced nephrotoxicity, which may be due in part to its antioxidant and anti-apoptotic effects, subsequently leading to improved renal function.
format Journal
author Sasivimon Promsan
Krit Jaikumkao
Anchalee Pongchaidecha
Nipon Chattipakorn
Varanuj Chatsudthipong
Phatchawan Arjinajarn
Wilart Pompimon
Anusorn Lungkaphin
author_facet Sasivimon Promsan
Krit Jaikumkao
Anchalee Pongchaidecha
Nipon Chattipakorn
Varanuj Chatsudthipong
Phatchawan Arjinajarn
Wilart Pompimon
Anusorn Lungkaphin
author_sort Sasivimon Promsan
title Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats
title_short Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats
title_full Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats
title_fullStr Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats
title_full_unstemmed Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats
title_sort pinocembrin attenuates gentamicin-induced nephrotoxicity in rats
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84979966329&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54097
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