Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III"

© 2015 Informa Healthcare USA, Inc. Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including oral and cervical cancers. Objective: To investigate anti-proliferative activity on human cervical (HeLa) and oral (KB) cancer cell lines of medicinal plants selected...

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Main Authors: Aranya Manosroi, Hiroyuki Akazawa, Kassara Pattamapun, Worapong Kitdamrongtham, Toshihiro Akihisa, Worapaka Manosroi, Jiradej Manosroi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/54137
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-541372018-09-04T10:24:58Z Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III" Aranya Manosroi Hiroyuki Akazawa Kassara Pattamapun Worapong Kitdamrongtham Toshihiro Akihisa Worapaka Manosroi Jiradej Manosroi Biochemistry, Genetics and Molecular Biology Medicine Pharmacology, Toxicology and Pharmaceutics © 2015 Informa Healthcare USA, Inc. Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including oral and cervical cancers. Objective: To investigate anti-proliferative activity on human cervical (HeLa) and oral (KB) cancer cell lines of medicinal plants selected from Thai/Lanna medicinal plant recipe database "MANOSROI III". Materials and methods: Twenty-three methanolic plant crude extracts were tested for phytochemicals and anti-proliferative activity on HeLa and KB cell lines for 24h by the sulforhodamine B (SRB) assay at the doses of 1×10-1×10-6mg/ml. The nine extracts with the concentrations giving 50% growth inhibition (GI50) lower than 100μg/ml were further semi-purified by liquid/liquid partition in order to evaluate and enhance the anti-proliferative potency. Results: All extracts contained steroids/triterpenoids, but not xanthones. The methanolic extracts of Gloriosa superba L. (Colchinaceae) root and Albizia chinensis (Osbeck) Merr. (Leguminosae-Mimosoideae) wood gave the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.91 (6.0-and 0.31-fold of cisplatin and doxorubicin) and 0.16μg/ml (28.78-and 82.29-fold of cisplatin and doxorubicin), respectively. Hexane and methanol-water fractions of G. superba exhibited the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.15 (37-and 1.9-fold of cisplatin and doxorubicin) and 0.058μg/ml (77.45-and 221.46-fold of cisplatin and doxorubicin), respectively. Discussion and conclusion: This study has demonstrated the potential of plants selected from MANOSROI III database especially G. superba and A. chinensis for further development as anti-oral and cervical cancer agents. 2018-09-04T10:08:10Z 2018-09-04T10:08:10Z 2015-07-01 Journal 17445116 13880209 2-s2.0-84930804593 10.3109/13880209.2014.959613 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930804593&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54137
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Aranya Manosroi
Hiroyuki Akazawa
Kassara Pattamapun
Worapong Kitdamrongtham
Toshihiro Akihisa
Worapaka Manosroi
Jiradej Manosroi
Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III"
description © 2015 Informa Healthcare USA, Inc. Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including oral and cervical cancers. Objective: To investigate anti-proliferative activity on human cervical (HeLa) and oral (KB) cancer cell lines of medicinal plants selected from Thai/Lanna medicinal plant recipe database "MANOSROI III". Materials and methods: Twenty-three methanolic plant crude extracts were tested for phytochemicals and anti-proliferative activity on HeLa and KB cell lines for 24h by the sulforhodamine B (SRB) assay at the doses of 1×10-1×10-6mg/ml. The nine extracts with the concentrations giving 50% growth inhibition (GI50) lower than 100μg/ml were further semi-purified by liquid/liquid partition in order to evaluate and enhance the anti-proliferative potency. Results: All extracts contained steroids/triterpenoids, but not xanthones. The methanolic extracts of Gloriosa superba L. (Colchinaceae) root and Albizia chinensis (Osbeck) Merr. (Leguminosae-Mimosoideae) wood gave the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.91 (6.0-and 0.31-fold of cisplatin and doxorubicin) and 0.16μg/ml (28.78-and 82.29-fold of cisplatin and doxorubicin), respectively. Hexane and methanol-water fractions of G. superba exhibited the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.15 (37-and 1.9-fold of cisplatin and doxorubicin) and 0.058μg/ml (77.45-and 221.46-fold of cisplatin and doxorubicin), respectively. Discussion and conclusion: This study has demonstrated the potential of plants selected from MANOSROI III database especially G. superba and A. chinensis for further development as anti-oral and cervical cancer agents.
format Journal
author Aranya Manosroi
Hiroyuki Akazawa
Kassara Pattamapun
Worapong Kitdamrongtham
Toshihiro Akihisa
Worapaka Manosroi
Jiradej Manosroi
author_facet Aranya Manosroi
Hiroyuki Akazawa
Kassara Pattamapun
Worapong Kitdamrongtham
Toshihiro Akihisa
Worapaka Manosroi
Jiradej Manosroi
author_sort Aranya Manosroi
title Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III"
title_short Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III"
title_full Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III"
title_fullStr Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III"
title_full_unstemmed Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "mANOSROI III"
title_sort potent anti-proliferative effects against oral and cervical cancers of thai medicinal plants selected from the thai/lanna medicinal plant recipe database "manosroi iii"
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930804593&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54137
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