Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR

© 2015, Malaysian Society for Parasitology. All rights reserved. Leishmaniasis is a neglected tropical parasitic disease affecting a large number of countries in the world. Early diagnosis of Leishmania infections is essential for therapeutic reasons, as it can decrease morbidity and mortality. L. s...

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Main Authors: S. Jungudomjaroen, A. Phumee, S. Chusri, K. Kraivichian, N. Jariyapan, S. Payungporn, P. Siriyasatien
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/54562
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-545622018-09-04T10:20:25Z Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR S. Jungudomjaroen A. Phumee S. Chusri K. Kraivichian N. Jariyapan S. Payungporn P. Siriyasatien Immunology and Microbiology Medicine © 2015, Malaysian Society for Parasitology. All rights reserved. Leishmaniasis is a neglected tropical parasitic disease affecting a large number of countries in the world. Early diagnosis of Leishmania infections is essential for therapeutic reasons, as it can decrease morbidity and mortality. L. siamensis and L. martiniquensis are novel Leishmania species recently described in Thailand and Myanmar. The disease is usually found in immunocompromised patients, especially those who have AIDS. Currently, the diagnosis of Leishmania infection in Thailand relies on microscopy, microbial culture, and polymerase chain reaction (PCR). In this study, we established a quantitative PCR (qPCR) method for detection of L. martiniquensis DNA in various types of clinical specimens, including whole blood, buffy coat, saliva, and urine of L. martiniquensis infected patients. The results of the qPCR assay were positive in all saliva samples. The assay is therefore effective to detect L. martiniquensis DNA even in noninvasive specimens, and it could be used for the diagnosis, follow up, and survey of L. martiniquensis infections. 2018-09-04T10:16:36Z 2018-09-04T10:16:36Z 2015-12-01 Journal 01275720 2-s2.0-84953870344 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84953870344&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54562
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
S. Jungudomjaroen
A. Phumee
S. Chusri
K. Kraivichian
N. Jariyapan
S. Payungporn
P. Siriyasatien
Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR
description © 2015, Malaysian Society for Parasitology. All rights reserved. Leishmaniasis is a neglected tropical parasitic disease affecting a large number of countries in the world. Early diagnosis of Leishmania infections is essential for therapeutic reasons, as it can decrease morbidity and mortality. L. siamensis and L. martiniquensis are novel Leishmania species recently described in Thailand and Myanmar. The disease is usually found in immunocompromised patients, especially those who have AIDS. Currently, the diagnosis of Leishmania infection in Thailand relies on microscopy, microbial culture, and polymerase chain reaction (PCR). In this study, we established a quantitative PCR (qPCR) method for detection of L. martiniquensis DNA in various types of clinical specimens, including whole blood, buffy coat, saliva, and urine of L. martiniquensis infected patients. The results of the qPCR assay were positive in all saliva samples. The assay is therefore effective to detect L. martiniquensis DNA even in noninvasive specimens, and it could be used for the diagnosis, follow up, and survey of L. martiniquensis infections.
format Journal
author S. Jungudomjaroen
A. Phumee
S. Chusri
K. Kraivichian
N. Jariyapan
S. Payungporn
P. Siriyasatien
author_facet S. Jungudomjaroen
A. Phumee
S. Chusri
K. Kraivichian
N. Jariyapan
S. Payungporn
P. Siriyasatien
author_sort S. Jungudomjaroen
title Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR
title_short Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR
title_full Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR
title_fullStr Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR
title_full_unstemmed Detection of Leishmania martiniquensis DNA in various clinical samples by quantitative PCR
title_sort detection of leishmania martiniquensis dna in various clinical samples by quantitative pcr
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84953870344&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54562
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