Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors

© 2015 Asian Journal of Transfusion Science. Context: Antibodies against human neutrophil antigens (HNAs) are implicated in immune-mediated neutropenia, transfusion-related acute lung injury and febrile transfusion reactions. Aims: This study aimed to determine HNA gene frequencies of the HNA-4 and...

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Main Authors: Onruedee Khantisitthiporn, Chollanot Kaset, Kamphon Intharanut, Nipapan Leetrakool, Oytip Nathalang
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/54766
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-547662018-09-04T10:23:01Z Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors Onruedee Khantisitthiporn Chollanot Kaset Kamphon Intharanut Nipapan Leetrakool Oytip Nathalang Medicine © 2015 Asian Journal of Transfusion Science. Context: Antibodies against human neutrophil antigens (HNAs) are implicated in immune-mediated neutropenia, transfusion-related acute lung injury and febrile transfusion reactions. Aims: This study aimed to determine HNA gene frequencies of the HNA-4 and HNA-5 systems among Thai populations and compare these frequencies with those previously reported for other populations. Materials and Methods: 800 DNA samples obtained from 500 unrelated healthy blood donors from Bangkok and 300 samples from Chiang Mai, Thailand were included. Samples were typed for each HNA allele including HNA-4a, HNA-4b, HNA-5a, and HNA-5b using an in-house polymerase chain reaction with sequence-specific primer technique. Results: The frequencies of HNA-4a and HNA-4b alleles in central Thais were 0.975 and 0.025, respectively and for Northern Thais, their frequencies were 0.965 and 0.035, respectively. For HNA-5a and HNA-5b alleles, their frequencies were 0.771 and 0.229; 0.748, and 0.252 in central and Northern Thais, respectively. The frequencies of HNA-4 and HNA-5 systems in central Thais are closely related to those in Northern Thais (P > 0.05). However, their frequencies were different from other populations (P < 0.001), except HNA-5a and HNA-5b gene frequencies in Thais were similar to Caucasians (P > 0.05). Conclusion: This study could contribute to predict the risk of alloimmunization to HNA-4 and HNA-5 systems, especially in feto-maternal incompatibility in Thais. 2018-09-04T10:23:01Z 2018-09-04T10:23:01Z 2015-01-01 Journal 19983565 09736247 2-s2.0-84939803741 10.4103/0973-6247.162699 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84939803741&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54766
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Onruedee Khantisitthiporn
Chollanot Kaset
Kamphon Intharanut
Nipapan Leetrakool
Oytip Nathalang
Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors
description © 2015 Asian Journal of Transfusion Science. Context: Antibodies against human neutrophil antigens (HNAs) are implicated in immune-mediated neutropenia, transfusion-related acute lung injury and febrile transfusion reactions. Aims: This study aimed to determine HNA gene frequencies of the HNA-4 and HNA-5 systems among Thai populations and compare these frequencies with those previously reported for other populations. Materials and Methods: 800 DNA samples obtained from 500 unrelated healthy blood donors from Bangkok and 300 samples from Chiang Mai, Thailand were included. Samples were typed for each HNA allele including HNA-4a, HNA-4b, HNA-5a, and HNA-5b using an in-house polymerase chain reaction with sequence-specific primer technique. Results: The frequencies of HNA-4a and HNA-4b alleles in central Thais were 0.975 and 0.025, respectively and for Northern Thais, their frequencies were 0.965 and 0.035, respectively. For HNA-5a and HNA-5b alleles, their frequencies were 0.771 and 0.229; 0.748, and 0.252 in central and Northern Thais, respectively. The frequencies of HNA-4 and HNA-5 systems in central Thais are closely related to those in Northern Thais (P > 0.05). However, their frequencies were different from other populations (P < 0.001), except HNA-5a and HNA-5b gene frequencies in Thais were similar to Caucasians (P > 0.05). Conclusion: This study could contribute to predict the risk of alloimmunization to HNA-4 and HNA-5 systems, especially in feto-maternal incompatibility in Thais.
format Journal
author Onruedee Khantisitthiporn
Chollanot Kaset
Kamphon Intharanut
Nipapan Leetrakool
Oytip Nathalang
author_facet Onruedee Khantisitthiporn
Chollanot Kaset
Kamphon Intharanut
Nipapan Leetrakool
Oytip Nathalang
author_sort Onruedee Khantisitthiporn
title Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors
title_short Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors
title_full Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors
title_fullStr Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors
title_full_unstemmed Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors
title_sort frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among thai blood donors
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84939803741&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54766
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