Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats

© 2015 The Japanese Society of Toxicologic Pathology. We previously established 3 cell lines (PLS10, PLS20 and PLS30) from a chemically-induced prostate carcinoma in F344 rats, and demonstrated high potential for metastasis in nude mice. In the present study, we investigated the feasibility of estab...

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Main Authors: Shugo Suzuki, Aya Naiki-Ito, Toshiya Kuno, Wanisa Punfa, Ne Long, Hiroyuki Kato, Shingo Inaguma, Masami Komiya, Tomoyuki Shirai, Satoru Takahashi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/54810
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-548102018-09-04T10:25:20Z Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats Shugo Suzuki Aya Naiki-Ito Toshiya Kuno Wanisa Punfa Ne Long Hiroyuki Kato Shingo Inaguma Masami Komiya Tomoyuki Shirai Satoru Takahashi Medicine Pharmacology, Toxicology and Pharmaceutics © 2015 The Japanese Society of Toxicologic Pathology. We previously established 3 cell lines (PLS10, PLS20 and PLS30) from a chemically-induced prostate carcinoma in F344 rats, and demonstrated high potential for metastasis in nude mice. In the present study, we investigated the feasibility of establishing an orthotopic model using the 3 rat prostate cancer cell lines in immunocompetent rats with the aim of resolving species-mismatch problems and defects of immune systems. The PLS10, PLS20 and PLS30 cell lines were injected into the ventral prostates of 6-weekold rats, which were then sacrificed at experimental weeks 4 and 8. Tumor mass formation was found in rats with PLS10, but not in those with PLS20 or PLS30. Additionally, metastatic carcinomas could be detected in lymph nodes and lungs of PLS10-inoculated rats. Genetic analysis demonstrated K-ras gene mutations in PLS10 and PLS20, but not in PLS30 cells. There were no mutations in p53 and KLF6. In conclusion, we established a syngeneic orthotopic model for prostate cancer in immunocompetent rats simulating human castration-resistant prostate cancer (CRPC), which should prove useful for development and validation of therapeutic agents, especially with immunotherapy. 2018-09-04T10:23:58Z 2018-09-04T10:23:58Z 2015-01-01 Journal 09149198 2-s2.0-84923368212 10.1293/tox.2014-0050 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84923368212&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54810
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Medicine
Pharmacology, Toxicology and Pharmaceutics
Shugo Suzuki
Aya Naiki-Ito
Toshiya Kuno
Wanisa Punfa
Ne Long
Hiroyuki Kato
Shingo Inaguma
Masami Komiya
Tomoyuki Shirai
Satoru Takahashi
Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats
description © 2015 The Japanese Society of Toxicologic Pathology. We previously established 3 cell lines (PLS10, PLS20 and PLS30) from a chemically-induced prostate carcinoma in F344 rats, and demonstrated high potential for metastasis in nude mice. In the present study, we investigated the feasibility of establishing an orthotopic model using the 3 rat prostate cancer cell lines in immunocompetent rats with the aim of resolving species-mismatch problems and defects of immune systems. The PLS10, PLS20 and PLS30 cell lines were injected into the ventral prostates of 6-weekold rats, which were then sacrificed at experimental weeks 4 and 8. Tumor mass formation was found in rats with PLS10, but not in those with PLS20 or PLS30. Additionally, metastatic carcinomas could be detected in lymph nodes and lungs of PLS10-inoculated rats. Genetic analysis demonstrated K-ras gene mutations in PLS10 and PLS20, but not in PLS30 cells. There were no mutations in p53 and KLF6. In conclusion, we established a syngeneic orthotopic model for prostate cancer in immunocompetent rats simulating human castration-resistant prostate cancer (CRPC), which should prove useful for development and validation of therapeutic agents, especially with immunotherapy.
format Journal
author Shugo Suzuki
Aya Naiki-Ito
Toshiya Kuno
Wanisa Punfa
Ne Long
Hiroyuki Kato
Shingo Inaguma
Masami Komiya
Tomoyuki Shirai
Satoru Takahashi
author_facet Shugo Suzuki
Aya Naiki-Ito
Toshiya Kuno
Wanisa Punfa
Ne Long
Hiroyuki Kato
Shingo Inaguma
Masami Komiya
Tomoyuki Shirai
Satoru Takahashi
author_sort Shugo Suzuki
title Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats
title_short Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats
title_full Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats
title_fullStr Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats
title_full_unstemmed Establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats
title_sort establishment of a syngeneic orthotopic model of prostate cancer in immunocompetent rats
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84923368212&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54810
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