A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta
The analysis of complex biological samples, principally biological fluids, to obtain global metabolite profiles using techniques such as high resolution NMR spectroscopy has been termed metabonomics. This work describes the application of metabonomics to characterize the biochemical changes associat...
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th-cmuir.6653943832-54932014-08-30T02:56:35Z A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta Phalaraksh C. Reynolds S.E. Wilson I.D. Lenz E.M. Nicholson J.K. Lindon J.C. The analysis of complex biological samples, principally biological fluids, to obtain global metabolite profiles using techniques such as high resolution NMR spectroscopy has been termed metabonomics. This work describes the application of metabonomics to characterize the biochemical changes associated with development during the various instars of the larval stages and in the pupal stage of the tobacco hornworm Manduca sexta. The levels of many small molecule metabolites change during development. Thus, alanine, glutamate, lysine, succinate, lactate, and betaine are present at relatively high levels during the feeding period of larval life, but decrease at the onset of wandering. Compounds that increase in concentration as the insects prepare to pupate include citrate, fatty acids, and succinate. The presence of variable levels of putrescine in haemolymph is interesting, given its possible role in juvenile hormone action. The potential of high resolution 1H-NMR spectroscopy for the metabonomic analysis of insect developmental chemistry is discussed. 2014-08-30T02:56:35Z 2014-08-30T02:56:35Z 2008 Article 15131874 10.2306/scienceasia1513-1874.2008.34.279 http://www.scopus.com/inward/record.url?eid=2-s2.0-53249149565&partnerID=40&md5=a385df0f70afac4a006d4e8e460fd115 http://cmuir.cmu.ac.th/handle/6653943832/5493 English |
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The analysis of complex biological samples, principally biological fluids, to obtain global metabolite profiles using techniques such as high resolution NMR spectroscopy has been termed metabonomics. This work describes the application of metabonomics to characterize the biochemical changes associated with development during the various instars of the larval stages and in the pupal stage of the tobacco hornworm Manduca sexta. The levels of many small molecule metabolites change during development. Thus, alanine, glutamate, lysine, succinate, lactate, and betaine are present at relatively high levels during the feeding period of larval life, but decrease at the onset of wandering. Compounds that increase in concentration as the insects prepare to pupate include citrate, fatty acids, and succinate. The presence of variable levels of putrescine in haemolymph is interesting, given its possible role in juvenile hormone action. The potential of high resolution 1H-NMR spectroscopy for the metabonomic analysis of insect developmental chemistry is discussed. |
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Article |
author |
Phalaraksh C. Reynolds S.E. Wilson I.D. Lenz E.M. Nicholson J.K. Lindon J.C. |
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Phalaraksh C. Reynolds S.E. Wilson I.D. Lenz E.M. Nicholson J.K. Lindon J.C. A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta |
author_facet |
Phalaraksh C. Reynolds S.E. Wilson I.D. Lenz E.M. Nicholson J.K. Lindon J.C. |
author_sort |
Phalaraksh C. |
title |
A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta |
title_short |
A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta |
title_full |
A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta |
title_fullStr |
A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta |
title_full_unstemmed |
A metabonomic analysis of insect development: 1H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta |
title_sort |
metabonomic analysis of insect development: 1h-nmr spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, manduca sexta |
publishDate |
2014 |
url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-53249149565&partnerID=40&md5=a385df0f70afac4a006d4e8e460fd115 http://cmuir.cmu.ac.th/handle/6653943832/5493 |
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