Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand

© 2016 Noroviruses (NoVs) have been recognized as a leading cause of sporadic cases and outbreaks of acute gastroenteritis in all age groups. During the surveillance of NoVs in Chiang Mai, Thailand, four cases of the novel GII.17 NoVs were sporadically detected by RT-PCR in 2014–2015. The first case...

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Main Authors: Pattara Khamrin, Kattareeya Kumthip, Arpaporn Yodmeeklin, Kanittapon Supadej, Nuthapong Ukarapol, Aksara Thongprachum, Shoko Okitsu, Satoshi Hayakawa, Hiroshi Ushijima, Niwat Maneekarn
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/54966
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-549662018-09-05T03:08:13Z Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand Pattara Khamrin Kattareeya Kumthip Arpaporn Yodmeeklin Kanittapon Supadej Nuthapong Ukarapol Aksara Thongprachum Shoko Okitsu Satoshi Hayakawa Hiroshi Ushijima Niwat Maneekarn Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine © 2016 Noroviruses (NoVs) have been recognized as a leading cause of sporadic cases and outbreaks of acute gastroenteritis in all age groups. During the surveillance of NoVs in Chiang Mai, Thailand, four cases of the novel GII.17 NoVs were sporadically detected by RT-PCR in 2014–2015. The first case of GII.17 was detected in a healthy adult who worked for a restaurant. The second case was found in a pediatric patient who admitted to the hospital with intussusception. The third and fourth cases were found in acute gastroenteritis children. Phylogenetic analysis clearly demonstrated that GII.17 NoVs detected in this study were genetically closely related with the novel GII.17 Kawasaki reference strains. These four GII.17 NoV positive specimens were also tested by two immunochromatographic test kits in order to evaluate the sensitivity for GII.17 NoV detection. The viral loads in those specimens were determined by real-time RT-PCR. The sensitivity of GII.17 NoV detection varies by individual test kits and also depending on the amount of the viruses contained in the fecal specimens. In summary, our study reported the detection of novel GII.17 NoVs in a wide range of subjects with and without diarrhea. Therefore, continued comprehensive screening and genetic molecular characterization of NoV strains circulating in this area need to be further investigated. 2018-09-05T02:50:29Z 2018-09-05T02:50:29Z 2016-10-01 Journal 15677257 15671348 2-s2.0-84979753445 10.1016/j.meegid.2016.07.031 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84979753445&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54966
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Pattara Khamrin
Kattareeya Kumthip
Arpaporn Yodmeeklin
Kanittapon Supadej
Nuthapong Ukarapol
Aksara Thongprachum
Shoko Okitsu
Satoshi Hayakawa
Hiroshi Ushijima
Niwat Maneekarn
Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand
description © 2016 Noroviruses (NoVs) have been recognized as a leading cause of sporadic cases and outbreaks of acute gastroenteritis in all age groups. During the surveillance of NoVs in Chiang Mai, Thailand, four cases of the novel GII.17 NoVs were sporadically detected by RT-PCR in 2014–2015. The first case of GII.17 was detected in a healthy adult who worked for a restaurant. The second case was found in a pediatric patient who admitted to the hospital with intussusception. The third and fourth cases were found in acute gastroenteritis children. Phylogenetic analysis clearly demonstrated that GII.17 NoVs detected in this study were genetically closely related with the novel GII.17 Kawasaki reference strains. These four GII.17 NoV positive specimens were also tested by two immunochromatographic test kits in order to evaluate the sensitivity for GII.17 NoV detection. The viral loads in those specimens were determined by real-time RT-PCR. The sensitivity of GII.17 NoV detection varies by individual test kits and also depending on the amount of the viruses contained in the fecal specimens. In summary, our study reported the detection of novel GII.17 NoVs in a wide range of subjects with and without diarrhea. Therefore, continued comprehensive screening and genetic molecular characterization of NoV strains circulating in this area need to be further investigated.
format Journal
author Pattara Khamrin
Kattareeya Kumthip
Arpaporn Yodmeeklin
Kanittapon Supadej
Nuthapong Ukarapol
Aksara Thongprachum
Shoko Okitsu
Satoshi Hayakawa
Hiroshi Ushijima
Niwat Maneekarn
author_facet Pattara Khamrin
Kattareeya Kumthip
Arpaporn Yodmeeklin
Kanittapon Supadej
Nuthapong Ukarapol
Aksara Thongprachum
Shoko Okitsu
Satoshi Hayakawa
Hiroshi Ushijima
Niwat Maneekarn
author_sort Pattara Khamrin
title Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand
title_short Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand
title_full Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand
title_fullStr Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand
title_full_unstemmed Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand
title_sort molecular characterization of norovirus gii.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84979753445&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54966
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