Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation

© 2016, Czech Academy of Agricultural Sciences. All rights reserved. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curve analy...

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Main Authors: Antonios Zambounis, Anastasios Samaras, Aliki Xanthopoulou, Maslin Osathanunkul, Leonardo Schena, Athanasios Tsaftaris, Panagiotis Madesis
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/55082
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spelling th-cmuir.6653943832-550822018-09-05T02:51:37Z Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation Antonios Zambounis Anastasios Samaras Aliki Xanthopoulou Maslin Osathanunkul Leonardo Schena Athanasios Tsaftaris Panagiotis Madesis Agricultural and Biological Sciences © 2016, Czech Academy of Agricultural Sciences. All rights reserved. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curve analysis of the amplicons specifically grouped all species into 10 respective unique and distinct HRM curve profiles. The analysis of the normalised HRM melting curves, assigning P. nicotianae as a normalised reference genotype, revealed that the genotype similarities among all the species were adequately low, indicating that Ypt1 marker was sufficient to identify and differentiate the tested species. This HRM method is rapid and reproducible allowing the identification of Phytophthora species and the screening of eventual variants eliminating the separate steps and reducing the risk of contamination. 2018-09-05T02:51:37Z 2018-09-05T02:51:37Z 2016-01-01 Journal 12122580 2-s2.0-84977138855 10.17221/179/2015-PPS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84977138855&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55082
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Antonios Zambounis
Anastasios Samaras
Aliki Xanthopoulou
Maslin Osathanunkul
Leonardo Schena
Athanasios Tsaftaris
Panagiotis Madesis
Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
description © 2016, Czech Academy of Agricultural Sciences. All rights reserved. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curve analysis of the amplicons specifically grouped all species into 10 respective unique and distinct HRM curve profiles. The analysis of the normalised HRM melting curves, assigning P. nicotianae as a normalised reference genotype, revealed that the genotype similarities among all the species were adequately low, indicating that Ypt1 marker was sufficient to identify and differentiate the tested species. This HRM method is rapid and reproducible allowing the identification of Phytophthora species and the screening of eventual variants eliminating the separate steps and reducing the risk of contamination.
format Journal
author Antonios Zambounis
Anastasios Samaras
Aliki Xanthopoulou
Maslin Osathanunkul
Leonardo Schena
Athanasios Tsaftaris
Panagiotis Madesis
author_facet Antonios Zambounis
Anastasios Samaras
Aliki Xanthopoulou
Maslin Osathanunkul
Leonardo Schena
Athanasios Tsaftaris
Panagiotis Madesis
author_sort Antonios Zambounis
title Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_short Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_full Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_fullStr Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_full_unstemmed Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_sort identification of phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84977138855&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55082
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