Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation

© 2016, Springer Science+Business Media Dordrecht. Due to the inconvenient and invasive nature of chondrocyte transplantation, preserved cartilage has been recognized as an alternative source of chondrocytes for implantation. However, there are major concerns, in particular, the viability and qualit...

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Main Authors: Wichaya Sriuttha, Nantawat Uttamo, Apisek Kongkaew, Jongkolnee Settakorn, Suchanan Rattanasalee, Prachya Kongtawelert, Dumnoensun Pruksakorn, Peraphan Pothacharoen
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/55120
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-551202018-09-05T03:07:31Z Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation Wichaya Sriuttha Nantawat Uttamo Apisek Kongkaew Jongkolnee Settakorn Suchanan Rattanasalee Prachya Kongtawelert Dumnoensun Pruksakorn Peraphan Pothacharoen Biochemistry, Genetics and Molecular Biology Engineering Materials Science Medicine © 2016, Springer Science+Business Media Dordrecht. Due to the inconvenient and invasive nature of chondrocyte transplantation, preserved cartilage has been recognized as an alternative source of chondrocytes for implantation. However, there are major concerns, in particular, the viability and quality of the chondrocytes. This study investigated the biochemistry and molecular characterization of chondrocytes isolated from preserved cartilage for purposes of transplantation. Ex vivo characterization was accomplished by storing human cartilage at either 4 or −80 °C in a preservation medium. Microscopic evaluation of the preserved cartilage was conducted after 1, 2, 3 and 6 weeks. The chondrocytes were isolated from the preserved cartilage and investigated for proliferation capacity and chondrogenic phenotype. Transplantation of chondrocytes from preserved cartilage into rabbit knees was performed for purposes of in vivo evaluation. The serum cartilage degradation biomarker (WF6 epitopes) was evaluated during the transplantation procedure. Human cartilage preserved for 1 week in a 10 % DMSO chondrogenic medium at 4 °C gave the highest chondrocyte viability. The isolated chondrocytes showed a high proliferative capacity and retained chondrogenic gene expression. Microscopic assessment of the implanted rabbit knees showed tissue regeneration and integration with the host cartilage. A decreased level of the serum biomarker after transplantation was evidence of in vivo repair by the implanted chondrocytes. These results suggest that cartilage preservation for 1 week in a 10 % DMSO chondrogenic medium at 4 °C can maintain proliferation capacity and the chondrogenic phenotype of human chondrocytes. These results can potentially be applied to in vivo allogeneic chondrocyte transplantation. Allogeneic chondrocytes from preserved cartilage would be expected to maintain their chondrogenic phenotype and to result in a high rate of success in transplanted grafts. 2018-09-05T02:52:01Z 2018-09-05T02:52:01Z 2016-12-01 Journal 15736814 13899333 2-s2.0-84982084104 10.1007/s10561-016-9577-2 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84982084104&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55120
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Engineering
Materials Science
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Engineering
Materials Science
Medicine
Wichaya Sriuttha
Nantawat Uttamo
Apisek Kongkaew
Jongkolnee Settakorn
Suchanan Rattanasalee
Prachya Kongtawelert
Dumnoensun Pruksakorn
Peraphan Pothacharoen
Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation
description © 2016, Springer Science+Business Media Dordrecht. Due to the inconvenient and invasive nature of chondrocyte transplantation, preserved cartilage has been recognized as an alternative source of chondrocytes for implantation. However, there are major concerns, in particular, the viability and quality of the chondrocytes. This study investigated the biochemistry and molecular characterization of chondrocytes isolated from preserved cartilage for purposes of transplantation. Ex vivo characterization was accomplished by storing human cartilage at either 4 or −80 °C in a preservation medium. Microscopic evaluation of the preserved cartilage was conducted after 1, 2, 3 and 6 weeks. The chondrocytes were isolated from the preserved cartilage and investigated for proliferation capacity and chondrogenic phenotype. Transplantation of chondrocytes from preserved cartilage into rabbit knees was performed for purposes of in vivo evaluation. The serum cartilage degradation biomarker (WF6 epitopes) was evaluated during the transplantation procedure. Human cartilage preserved for 1 week in a 10 % DMSO chondrogenic medium at 4 °C gave the highest chondrocyte viability. The isolated chondrocytes showed a high proliferative capacity and retained chondrogenic gene expression. Microscopic assessment of the implanted rabbit knees showed tissue regeneration and integration with the host cartilage. A decreased level of the serum biomarker after transplantation was evidence of in vivo repair by the implanted chondrocytes. These results suggest that cartilage preservation for 1 week in a 10 % DMSO chondrogenic medium at 4 °C can maintain proliferation capacity and the chondrogenic phenotype of human chondrocytes. These results can potentially be applied to in vivo allogeneic chondrocyte transplantation. Allogeneic chondrocytes from preserved cartilage would be expected to maintain their chondrogenic phenotype and to result in a high rate of success in transplanted grafts.
format Journal
author Wichaya Sriuttha
Nantawat Uttamo
Apisek Kongkaew
Jongkolnee Settakorn
Suchanan Rattanasalee
Prachya Kongtawelert
Dumnoensun Pruksakorn
Peraphan Pothacharoen
author_facet Wichaya Sriuttha
Nantawat Uttamo
Apisek Kongkaew
Jongkolnee Settakorn
Suchanan Rattanasalee
Prachya Kongtawelert
Dumnoensun Pruksakorn
Peraphan Pothacharoen
author_sort Wichaya Sriuttha
title Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation
title_short Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation
title_full Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation
title_fullStr Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation
title_full_unstemmed Ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation
title_sort ex vivo and in vivo characterization of cold preserved cartilage for cell transplantation
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84982084104&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55120
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