Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease

• Main Authors: Kanet Pakdeepak, Supansa Pata, Sawitree Chiampanichayakul, Watchara Kasinrerk, Thanusak Tatu
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Health Professions; Immunology and Microbiology; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84987958839&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/55129
• Institution: Chiang Mai University

© 2016 Taylor & Francis. Monoclonal antibodies against α-globin containing human Hbs, named AMS-Alpha1 and AMS-Alpha 2, were produced by the hybridoma technique using spleen cells enriched by the newly developed B lymphocyte enrichment protocol. These two monoclonal antibodies were of IgM class, reacting to only intact form of human Hbs A, A2, E, and F, which contain α-globin chain. By the indirect ELISA, the AMS-Alpha1 and AMS-Alpha 2 quantified less amount of α-globin chain containing hemoglobins in HbH disease than the SEA-α thalassemia 1 carriers and normal individuals. It was thus anticipated that these monoclonal antibodies can be used for detecting Hb Bart’s hydrops fetalis in which no α-globin chain is produced.