A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD

© 2016 Elsevier B.V. Organophosphate (OP) pesticides are widely used for crop protection in many countries including Thailand. Aside from causing environmental contamination, they affect human health especially by over-stimulating of the neurotransmission system. OP pesticides, as with other non-per...

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Main Authors: Warangkana Naksen, Tippawan Prapamontol, Ampica Mangklabruks, Somporn Chantara, Prasak Thavornyutikarn, Mark G. Robson, P. Barry Ryan, Dana Boyd Barr, Parinya Panuwet
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/55191
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-551912018-09-05T02:56:09Z A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD Warangkana Naksen Tippawan Prapamontol Ampica Mangklabruks Somporn Chantara Prasak Thavornyutikarn Mark G. Robson P. Barry Ryan Dana Boyd Barr Parinya Panuwet Biochemistry, Genetics and Molecular Biology Chemistry © 2016 Elsevier B.V. Organophosphate (OP) pesticides are widely used for crop protection in many countries including Thailand. Aside from causing environmental contamination, they affect human health especially by over-stimulating of the neurotransmission system. OP pesticides, as with other non-persistent pesticides, degrade quickly in the environment as well as are metabolized quite rapidly in humans. Assessing human exposures to these compounds requires analytical methods that are sensitive, robust, and most importantly, suitable for specific laboratory settings. The aim of this study was to develop and validate an analytical method for measuring 11 OP pesticide residues in human plasma and breast milk. Analytes in both plasma and breast milk samples were extracted with acetone and methylene chloride, cleaned-up using aminopropyl solid phase extraction cartridges, and analyzed by gas chromatography with flame photometric detection. The optimized method exhibited good linearity, with the coefficients of determination of 0.996-0.999 and <7% error about the slope. Extraction recoveries from spiked plasma and breast milk samples at low and medium concentrations (0.8-5.0 and 1.6-10 ng mL-1, respectively) ranged from 59.4% (ethion) to 94.0% (chlorpyrifos). Intra-batch and inter-batch precisions ranged from 2.3-18.9% and 5.8-19.5%, respectively. Method detection limits of plasma and breast milk ranged from 0.18-1.36 and 0.09-2.66 ng mL-1, respectively. We analyzed 63 plasma and 30 breastmilk samples collected from farmworkers in Chiang Mai Province to determine the suitability of this method for occupational exposure assessment. Of the 11 pesticides measured, seven were detected in plasma samples and five were detected in breast milk samples. Mass spectrometry was used to confirm results. Overall, this method is rapid and reliable. It offers the laboratories with limited access to mass spectrometry a capacity to investigate levels OP pesticides in plasma and breastmilk in those occupationally exposed for health risk assessment. 2018-09-05T02:52:54Z 2018-09-05T02:52:54Z 2016-07-01 Journal 1873376X 15700232 2-s2.0-84969593631 10.1016/j.jchromb.2016.04.045 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84969593631&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55191
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Warangkana Naksen
Tippawan Prapamontol
Ampica Mangklabruks
Somporn Chantara
Prasak Thavornyutikarn
Mark G. Robson
P. Barry Ryan
Dana Boyd Barr
Parinya Panuwet
A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD
description © 2016 Elsevier B.V. Organophosphate (OP) pesticides are widely used for crop protection in many countries including Thailand. Aside from causing environmental contamination, they affect human health especially by over-stimulating of the neurotransmission system. OP pesticides, as with other non-persistent pesticides, degrade quickly in the environment as well as are metabolized quite rapidly in humans. Assessing human exposures to these compounds requires analytical methods that are sensitive, robust, and most importantly, suitable for specific laboratory settings. The aim of this study was to develop and validate an analytical method for measuring 11 OP pesticide residues in human plasma and breast milk. Analytes in both plasma and breast milk samples were extracted with acetone and methylene chloride, cleaned-up using aminopropyl solid phase extraction cartridges, and analyzed by gas chromatography with flame photometric detection. The optimized method exhibited good linearity, with the coefficients of determination of 0.996-0.999 and <7% error about the slope. Extraction recoveries from spiked plasma and breast milk samples at low and medium concentrations (0.8-5.0 and 1.6-10 ng mL-1, respectively) ranged from 59.4% (ethion) to 94.0% (chlorpyrifos). Intra-batch and inter-batch precisions ranged from 2.3-18.9% and 5.8-19.5%, respectively. Method detection limits of plasma and breast milk ranged from 0.18-1.36 and 0.09-2.66 ng mL-1, respectively. We analyzed 63 plasma and 30 breastmilk samples collected from farmworkers in Chiang Mai Province to determine the suitability of this method for occupational exposure assessment. Of the 11 pesticides measured, seven were detected in plasma samples and five were detected in breast milk samples. Mass spectrometry was used to confirm results. Overall, this method is rapid and reliable. It offers the laboratories with limited access to mass spectrometry a capacity to investigate levels OP pesticides in plasma and breastmilk in those occupationally exposed for health risk assessment.
format Journal
author Warangkana Naksen
Tippawan Prapamontol
Ampica Mangklabruks
Somporn Chantara
Prasak Thavornyutikarn
Mark G. Robson
P. Barry Ryan
Dana Boyd Barr
Parinya Panuwet
author_facet Warangkana Naksen
Tippawan Prapamontol
Ampica Mangklabruks
Somporn Chantara
Prasak Thavornyutikarn
Mark G. Robson
P. Barry Ryan
Dana Boyd Barr
Parinya Panuwet
author_sort Warangkana Naksen
title A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD
title_short A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD
title_full A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD
title_fullStr A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD
title_full_unstemmed A single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using GC-FPD
title_sort single method for detecting 11 organophosphate pesticides in human plasma and breastmilk using gc-fpd
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84969593631&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55191
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