In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts
© 2016, The Society for In Vitro Biology. Thirty-one dichloromethane and methanol crude extracts of 16 herb species used in Thai traditional folk medicine were studied for their cytotoxic activities on the SW 1353 chondrosarcoma cell line and primary chondrocytes. Methyl thiazolyl tetrazolium (MTT)...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84957697867&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55221 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-55221 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-552212018-09-05T02:53:15Z In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts Napat Ruamrungsri Puntita Siengdee Korawan Sringarm Siriwadee Chomdej Siriwan Ongchai Korakot Nganvongpanit Biochemistry, Genetics and Molecular Biology © 2016, The Society for In Vitro Biology. Thirty-one dichloromethane and methanol crude extracts of 16 herb species used in Thai traditional folk medicine were studied for their cytotoxic activities on the SW 1353 chondrosarcoma cell line and primary chondrocytes. Methyl thiazolyl tetrazolium (MTT) cell viability assay and flow cytometric method were used as screening tools for cytotoxicity testing. The half maximal inhibitory concentration (IC50) was measured and reported for each crude extract. Apoptosis, necrosis, and cell viability were measured by flow cytometry at IC50.Two out of 31 herbal extracts, methanol extracts of Paris polyphylla var. chinensis and Ficus thailandica C.C. Berg & S. Gardner, showed potent anticancer activity. They demonstrated high apoptosis induction activity in SW 1353 cells but had less effect on percentage of viability and necrosis of normal chondrocyte cells. Cytotoxic screening and apoptosis assays suggest the potential anticancer activity of some plants used in Thai traditional medicine and provide information concerning their direct effects. 2018-09-05T02:53:15Z 2018-09-05T02:53:15Z 2016-04-01 Journal 10712690 2-s2.0-84957697867 10.1007/s11626-016-0006-4 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84957697867&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55221 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
topic |
Biochemistry, Genetics and Molecular Biology |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Napat Ruamrungsri Puntita Siengdee Korawan Sringarm Siriwadee Chomdej Siriwan Ongchai Korakot Nganvongpanit In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts |
description |
© 2016, The Society for In Vitro Biology. Thirty-one dichloromethane and methanol crude extracts of 16 herb species used in Thai traditional folk medicine were studied for their cytotoxic activities on the SW 1353 chondrosarcoma cell line and primary chondrocytes. Methyl thiazolyl tetrazolium (MTT) cell viability assay and flow cytometric method were used as screening tools for cytotoxicity testing. The half maximal inhibitory concentration (IC50) was measured and reported for each crude extract. Apoptosis, necrosis, and cell viability were measured by flow cytometry at IC50.Two out of 31 herbal extracts, methanol extracts of Paris polyphylla var. chinensis and Ficus thailandica C.C. Berg & S. Gardner, showed potent anticancer activity. They demonstrated high apoptosis induction activity in SW 1353 cells but had less effect on percentage of viability and necrosis of normal chondrocyte cells. Cytotoxic screening and apoptosis assays suggest the potential anticancer activity of some plants used in Thai traditional medicine and provide information concerning their direct effects. |
format |
Journal |
author |
Napat Ruamrungsri Puntita Siengdee Korawan Sringarm Siriwadee Chomdej Siriwan Ongchai Korakot Nganvongpanit |
author_facet |
Napat Ruamrungsri Puntita Siengdee Korawan Sringarm Siriwadee Chomdej Siriwan Ongchai Korakot Nganvongpanit |
author_sort |
Napat Ruamrungsri |
title |
In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts |
title_short |
In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts |
title_full |
In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts |
title_fullStr |
In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts |
title_full_unstemmed |
In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts |
title_sort |
in vitro cytotoxic screening of 31 crude extracts of thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84957697867&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55221 |
_version_ |
1681424465541464064 |