Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats

© by Lab & Life Press/SBDR. OBJECTIVES: Diabetes and exercise training have been shown to involve interleukin 6 (IL-6) signaling in muscle. However, the relationship between the actions of these two stimuli on muscle IL-6 and their downstream components is still unknown. Thus, the effect of en...

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Main Authors: Pattarawan Pattamaprapanont, Chatchai Muanprasat, Sunhapas Soodvilai, Chutima Srimaroeng, Varanuj Chatsudthipong
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/55279
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spelling th-cmuir.6653943832-552792018-09-05T03:10:58Z Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats Pattarawan Pattamaprapanont Chatchai Muanprasat Sunhapas Soodvilai Chutima Srimaroeng Varanuj Chatsudthipong Biochemistry, Genetics and Molecular Biology Medicine © by Lab & Life Press/SBDR. OBJECTIVES: Diabetes and exercise training have been shown to involve interleukin 6 (IL-6) signaling in muscle. However, the relationship between the actions of these two stimuli on muscle IL-6 and their downstream components is still unknown. Thus, the effect of endurance training on the key components of muscle IL-6 signaling transduction was investigated in a rat model of type 2 diabetes. METHODS: Diabetes was induced by streptozotocin (STZ) in male Wistar rats fed a high-fat diet, with normal rats acting as controls. The animals were left to conduct their normal activities or assigned to endurance training in a treadmill. At the end of 8 weeks, blood biochemical profiles, exercise performance, muscle oxidative capacity, glucose transporter 4 (GLUT4) protein distribution, and expressions of IL-6 and its downstream proteins were determined. RESULTS: Blood biochemical profiles of the diabetic rats were altered compared to normal rats, whereas endurance training improved blood chemistry and exercise performance. It also increased muscle oxidative capacity, and promoted GLUT4 subcellular localization to the membrane in muscles. Furthermore, protein expression of IL-6 receptor (IL-6Rα) was increased in both normal and diabetic rats after endurance training, but no significant changes in IL-6, phosphorylated signal transducer and activator of transcription 3 (p-STAT3), or suppressor of cytokine signaling 3 (SOC3) were observed in muscles of normal and diabetic rats. CONCLUSIONS: IL-6 signaling pathway mediating muscle response to endurance training was conserved in type 2 diabetes. There was no link between training-induced IL-6 downstream targets in skeletal muscles and IL-6-induced type 2 diabetes. 2018-09-05T02:53:56Z 2018-09-05T02:53:56Z 2016-01-01 Journal 16130575 16136071 2-s2.0-85007499920 10.1900/RDS.2016.13.197 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85007499920&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55279
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Pattarawan Pattamaprapanont
Chatchai Muanprasat
Sunhapas Soodvilai
Chutima Srimaroeng
Varanuj Chatsudthipong
Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats
description © by Lab & Life Press/SBDR. OBJECTIVES: Diabetes and exercise training have been shown to involve interleukin 6 (IL-6) signaling in muscle. However, the relationship between the actions of these two stimuli on muscle IL-6 and their downstream components is still unknown. Thus, the effect of endurance training on the key components of muscle IL-6 signaling transduction was investigated in a rat model of type 2 diabetes. METHODS: Diabetes was induced by streptozotocin (STZ) in male Wistar rats fed a high-fat diet, with normal rats acting as controls. The animals were left to conduct their normal activities or assigned to endurance training in a treadmill. At the end of 8 weeks, blood biochemical profiles, exercise performance, muscle oxidative capacity, glucose transporter 4 (GLUT4) protein distribution, and expressions of IL-6 and its downstream proteins were determined. RESULTS: Blood biochemical profiles of the diabetic rats were altered compared to normal rats, whereas endurance training improved blood chemistry and exercise performance. It also increased muscle oxidative capacity, and promoted GLUT4 subcellular localization to the membrane in muscles. Furthermore, protein expression of IL-6 receptor (IL-6Rα) was increased in both normal and diabetic rats after endurance training, but no significant changes in IL-6, phosphorylated signal transducer and activator of transcription 3 (p-STAT3), or suppressor of cytokine signaling 3 (SOC3) were observed in muscles of normal and diabetic rats. CONCLUSIONS: IL-6 signaling pathway mediating muscle response to endurance training was conserved in type 2 diabetes. There was no link between training-induced IL-6 downstream targets in skeletal muscles and IL-6-induced type 2 diabetes.
format Journal
author Pattarawan Pattamaprapanont
Chatchai Muanprasat
Sunhapas Soodvilai
Chutima Srimaroeng
Varanuj Chatsudthipong
author_facet Pattarawan Pattamaprapanont
Chatchai Muanprasat
Sunhapas Soodvilai
Chutima Srimaroeng
Varanuj Chatsudthipong
author_sort Pattarawan Pattamaprapanont
title Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats
title_short Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats
title_full Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats
title_fullStr Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats
title_full_unstemmed Effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats
title_sort effect of exercise training on signaling of interleukin-6 in skeletal muscles of type 2 diabetic rats
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85007499920&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55279
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