The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells

© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background/Aim: Tooth avulsion causes an injury to the periodontal ligament (PDL). The success of tooth replantation depends on the quantity and quality of PDL cells. The aim of this study was to examine the preservative an...

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Main Authors: Attaporn Prueksakorn, Subin Puasiri, Supanigar Ruangsri, Anupong Makeudom, Thanapat Sastraruji, Suttichai Krisanaprakornkit, Pattama Chailertvanitkul
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/55619
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spelling th-cmuir.6653943832-556192018-09-05T02:58:54Z The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells Attaporn Prueksakorn Subin Puasiri Supanigar Ruangsri Anupong Makeudom Thanapat Sastraruji Suttichai Krisanaprakornkit Pattama Chailertvanitkul Dentistry © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background/Aim: Tooth avulsion causes an injury to the periodontal ligament (PDL). The success of tooth replantation depends on the quantity and quality of PDL cells. The aim of this study was to examine the preservative and proliferative effects of Thai propolis extract, previously shown to exert anti-inflammatory and antioxidant activities, on human PDL cells. Materials and methods: Ninety-six premolars were left to air dry for 30 min and stored in Hank's balanced salt solution (HBSS), milk, or various concentrations of propolis extract from 0.25 to 10 mg ml−1for 3 h. PDL cells were isolated by collagenase and trypsin digestion, and their viability was determined by a trypan blue dye exclusion assay. PDL tissues were also scraped off the root surface and cultured to determine cell growth and morphology. The alamarBlue®and BrdU assays were performed to determine the cytotoxic and proliferative effects of the extract on cultured PDL cells, respectively. Results: A non-toxic dose of 2.5 mg ml−1of propolis extract yielded the greatest percentage of cell viability (78.84 ± 3.34%), which was significantly higher than those of the other concentrations (P < 0.001). Nevertheless, this percentage was not significantly different from that of HBSS (80.14 ± 2.44%; P = 1.00), but was significantly higher than that of milk (71.27 ± 2.79%; P < 0.001). The cells grown from PDL explants looked like fibroblasts. However, 2.5 mg ml−1of the extract did not induce PDL cell proliferation. Conclusion: Thai propolis extract at 2.5 mg ml−1appears to be the most effective dose for preserving the viability of PDL cells, and this was comparable to HBSS. 2018-09-05T02:58:54Z 2018-09-05T02:58:54Z 2016-12-01 Journal 16009657 16004469 2-s2.0-84979025625 10.1111/edt.12292 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84979025625&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55619
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Dentistry
spellingShingle Dentistry
Attaporn Prueksakorn
Subin Puasiri
Supanigar Ruangsri
Anupong Makeudom
Thanapat Sastraruji
Suttichai Krisanaprakornkit
Pattama Chailertvanitkul
The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells
description © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background/Aim: Tooth avulsion causes an injury to the periodontal ligament (PDL). The success of tooth replantation depends on the quantity and quality of PDL cells. The aim of this study was to examine the preservative and proliferative effects of Thai propolis extract, previously shown to exert anti-inflammatory and antioxidant activities, on human PDL cells. Materials and methods: Ninety-six premolars were left to air dry for 30 min and stored in Hank's balanced salt solution (HBSS), milk, or various concentrations of propolis extract from 0.25 to 10 mg ml−1for 3 h. PDL cells were isolated by collagenase and trypsin digestion, and their viability was determined by a trypan blue dye exclusion assay. PDL tissues were also scraped off the root surface and cultured to determine cell growth and morphology. The alamarBlue®and BrdU assays were performed to determine the cytotoxic and proliferative effects of the extract on cultured PDL cells, respectively. Results: A non-toxic dose of 2.5 mg ml−1of propolis extract yielded the greatest percentage of cell viability (78.84 ± 3.34%), which was significantly higher than those of the other concentrations (P < 0.001). Nevertheless, this percentage was not significantly different from that of HBSS (80.14 ± 2.44%; P = 1.00), but was significantly higher than that of milk (71.27 ± 2.79%; P < 0.001). The cells grown from PDL explants looked like fibroblasts. However, 2.5 mg ml−1of the extract did not induce PDL cell proliferation. Conclusion: Thai propolis extract at 2.5 mg ml−1appears to be the most effective dose for preserving the viability of PDL cells, and this was comparable to HBSS.
format Journal
author Attaporn Prueksakorn
Subin Puasiri
Supanigar Ruangsri
Anupong Makeudom
Thanapat Sastraruji
Suttichai Krisanaprakornkit
Pattama Chailertvanitkul
author_facet Attaporn Prueksakorn
Subin Puasiri
Supanigar Ruangsri
Anupong Makeudom
Thanapat Sastraruji
Suttichai Krisanaprakornkit
Pattama Chailertvanitkul
author_sort Attaporn Prueksakorn
title The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells
title_short The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells
title_full The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells
title_fullStr The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells
title_full_unstemmed The preservative effect of Thai propolis extract on the viability of human periodontal ligament cells
title_sort preservative effect of thai propolis extract on the viability of human periodontal ligament cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84979025625&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55619
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