Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract

© 2016 The Author(s). Background: Natural products made from plant sources have been used in a variety of cosmetic applications as a source of nutrition and as a whitening agent. The flowers of Cassia fistula L, family Fabaceae, have been used as a traditional medicine for skin diseases and wound he...

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Main Authors: Pornngarm Limtrakul, Supachai Yodkeeree, Pilaiporn Thippraphan, Wanisa Punfa, Jatupol Srisomboon
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/55990
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-559902018-09-05T03:07:26Z Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract Pornngarm Limtrakul Supachai Yodkeeree Pilaiporn Thippraphan Wanisa Punfa Jatupol Srisomboon Medicine © 2016 The Author(s). Background: Natural products made from plant sources have been used in a variety of cosmetic applications as a source of nutrition and as a whitening agent. The flowers of Cassia fistula L, family Fabaceae, have been used as a traditional medicine for skin diseases and wound healing and have been reported to possess anti-oxidant properties. The anti-aging effect of C. fistula flower extract on human skin fibroblast was investigated. Methods: The butanolic extraction of C. fistula flowers was completed and the active compounds were classified. The cytotoxicity of fibroblasts was evaluated by SRB assay for the purposes of selecting non-toxic doses for further experiments. The collagen and hyaluronic acid (HA) synthesis was then measured using the collagen kit and ELISA, respectively. Moreover, the enzyme activity, including collagenase, matrixmelloproteinase-2 (MMP-2) and tyrosinase, were also evaluated. Results: It was found that the flower extract did not affect skin fibroblast cell growth (IC50> 200 μg/mL). The results did show that the flower extract significantly increased collagen and HA synthesis in a dose dependent manner. The flower extract (50-200 μg/mL) also significantly inhibited collagenase and MMP-2 activity. Furthermore, this flower extract could inhibit the tyrosinase activity that causes hyperpigmentation, which induces skin aging. Conclusions: The C. fistula flower extract displayed a preventive effect when used for anti-aging purposes in human skin fibroblasts and may be an appropriate choice for cosmetic products that aim to provide whitening effects, and which are designated as anti-aging facial skin care products. 2018-09-05T03:07:26Z 2018-09-05T03:07:26Z 2016-12-03 Journal 14726882 2-s2.0-85000925704 10.1186/s12906-016-1484-3 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85000925704&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55990
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Pornngarm Limtrakul
Supachai Yodkeeree
Pilaiporn Thippraphan
Wanisa Punfa
Jatupol Srisomboon
Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
description © 2016 The Author(s). Background: Natural products made from plant sources have been used in a variety of cosmetic applications as a source of nutrition and as a whitening agent. The flowers of Cassia fistula L, family Fabaceae, have been used as a traditional medicine for skin diseases and wound healing and have been reported to possess anti-oxidant properties. The anti-aging effect of C. fistula flower extract on human skin fibroblast was investigated. Methods: The butanolic extraction of C. fistula flowers was completed and the active compounds were classified. The cytotoxicity of fibroblasts was evaluated by SRB assay for the purposes of selecting non-toxic doses for further experiments. The collagen and hyaluronic acid (HA) synthesis was then measured using the collagen kit and ELISA, respectively. Moreover, the enzyme activity, including collagenase, matrixmelloproteinase-2 (MMP-2) and tyrosinase, were also evaluated. Results: It was found that the flower extract did not affect skin fibroblast cell growth (IC50> 200 μg/mL). The results did show that the flower extract significantly increased collagen and HA synthesis in a dose dependent manner. The flower extract (50-200 μg/mL) also significantly inhibited collagenase and MMP-2 activity. Furthermore, this flower extract could inhibit the tyrosinase activity that causes hyperpigmentation, which induces skin aging. Conclusions: The C. fistula flower extract displayed a preventive effect when used for anti-aging purposes in human skin fibroblasts and may be an appropriate choice for cosmetic products that aim to provide whitening effects, and which are designated as anti-aging facial skin care products.
format Journal
author Pornngarm Limtrakul
Supachai Yodkeeree
Pilaiporn Thippraphan
Wanisa Punfa
Jatupol Srisomboon
author_facet Pornngarm Limtrakul
Supachai Yodkeeree
Pilaiporn Thippraphan
Wanisa Punfa
Jatupol Srisomboon
author_sort Pornngarm Limtrakul
title Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
title_short Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
title_full Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
title_fullStr Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
title_full_unstemmed Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
title_sort anti-aging and tyrosinase inhibition effects of cassia fistula flower butanolic extract
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85000925704&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55990
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