Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1

© 2016 Arunee Thanasarasakulpong et al. Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immun...

Full description

Saved in:
Bibliographic Details
Main Authors: Arunee Thanasarasakulpong, Pichayanut Poolperm, Weerapongse Tangjitjaroen, Thanya Varinrak, Takuo Sawada, Dirk Pfeiffer, Nattawooti Sthitmatee
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958581641&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56346
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-56346
record_format dspace
spelling th-cmuir.6653943832-563462018-09-05T03:15:18Z Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1 Arunee Thanasarasakulpong Pichayanut Poolperm Weerapongse Tangjitjaroen Thanya Varinrak Takuo Sawada Dirk Pfeiffer Nattawooti Sthitmatee Veterinary © 2016 Arunee Thanasarasakulpong et al. Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immunogenicity of rOmpH purified using electroelution with rOmpH purified using affinity chromatography and native OmpH purified using electroelution and a nonimmunized control group. Chickens immunized with rOmpH purified using electroelution produced the highest ELISA antibody levels against P. multocida strains. Chickens in each of the 5 treatment groups were split into two subgroups for challenge with two different P. multocida strains. The average number of adhesions to CEF cells was statistically significantly lower in sera from chickens immunized with rOmpH or native OmpH purified using electroelution than in those of the three other treatment groups. The survival amongst chickens immunized with rOmpH or native OmpH purified using electroelution indicated high levels of protection. In contrast, survival probability was zero or low in the groups immunized with rOmpH purified using affinity chromatography and in the nonimmunized group. These findings show that the rOmpH purified using electroelution retains its immunogenicity and stimulates high levels of protection in chickens against P. multocida infection. 2018-09-05T03:15:18Z 2018-09-05T03:15:18Z 2016-01-01 Journal 20420048 2-s2.0-84958581641 10.1155/2016/2579345 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958581641&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56346
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Veterinary
spellingShingle Veterinary
Arunee Thanasarasakulpong
Pichayanut Poolperm
Weerapongse Tangjitjaroen
Thanya Varinrak
Takuo Sawada
Dirk Pfeiffer
Nattawooti Sthitmatee
Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
description © 2016 Arunee Thanasarasakulpong et al. Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immunogenicity of rOmpH purified using electroelution with rOmpH purified using affinity chromatography and native OmpH purified using electroelution and a nonimmunized control group. Chickens immunized with rOmpH purified using electroelution produced the highest ELISA antibody levels against P. multocida strains. Chickens in each of the 5 treatment groups were split into two subgroups for challenge with two different P. multocida strains. The average number of adhesions to CEF cells was statistically significantly lower in sera from chickens immunized with rOmpH or native OmpH purified using electroelution than in those of the three other treatment groups. The survival amongst chickens immunized with rOmpH or native OmpH purified using electroelution indicated high levels of protection. In contrast, survival probability was zero or low in the groups immunized with rOmpH purified using affinity chromatography and in the nonimmunized group. These findings show that the rOmpH purified using electroelution retains its immunogenicity and stimulates high levels of protection in chickens against P. multocida infection.
format Journal
author Arunee Thanasarasakulpong
Pichayanut Poolperm
Weerapongse Tangjitjaroen
Thanya Varinrak
Takuo Sawada
Dirk Pfeiffer
Nattawooti Sthitmatee
author_facet Arunee Thanasarasakulpong
Pichayanut Poolperm
Weerapongse Tangjitjaroen
Thanya Varinrak
Takuo Sawada
Dirk Pfeiffer
Nattawooti Sthitmatee
author_sort Arunee Thanasarasakulpong
title Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_short Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_full Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_fullStr Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_full_unstemmed Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_sort comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein h of pasteurella multocida serovar a:1
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958581641&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56346
_version_ 1681424674854010880