Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions

The Chinese citrus fruit fly, Bactrocera minax, is an economically important pest of citrus. It exhibits pupal diapause from November to May to combat harsh environmental conditions. Such a long pupal diapause is a barrier for laboratory rearing and development of control strategies against this pes...

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Main Authors: Zhenzhong Chen, Yongcheng Dong, Yaohui Wang, Awawing A. Andongma, Muhammad A. Rashid, Patcharin Krutmuang, Changying Niu
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/56358
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-563582018-09-05T03:15:26Z Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions Zhenzhong Chen Yongcheng Dong Yaohui Wang Awawing A. Andongma Muhammad A. Rashid Patcharin Krutmuang Changying Niu Multidisciplinary The Chinese citrus fruit fly, Bactrocera minax, is an economically important pest of citrus. It exhibits pupal diapause from November to May to combat harsh environmental conditions. Such a long pupal diapause is a barrier for laboratory rearing and development of control strategies against this pest. In the present study, 20-hydroxyecdysone (20E) was used to break pupal diapause of B. minax by topical application. After diapause termination by 20E treated, the pupal ontogenetic processes were observed along the temporal trajectory. The pupal response time to 20E was estimated by detecting the relative expression of 20E responsive genes at different times after 20E-treatment. Results revealed that 20E could effectively terminate the pupal diapause in a dose-dependent manner and significantly shorten the time for 50% adult emergence (Et 50). 20E response genes, including ecr, broad and foxo, were up-regulated within 72h, indicating these genes are involved in pupal metamorphosis and diapause termination processes. Morphological changes showed the pupal metamorphosis began ∼7 days after 20E-treatment at 22 °C. This study does not only pave the way for artificial rearing in the laboratory through manipulating of pupal diapause termination, but also deepens our understanding of the underlying pupal diapause termination mechanism of B. minax. 2018-09-05T03:15:26Z 2018-09-05T03:15:26Z 2016-06-08 Journal 20452322 2-s2.0-84976469659 10.1038/srep27440 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84976469659&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56358
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Zhenzhong Chen
Yongcheng Dong
Yaohui Wang
Awawing A. Andongma
Muhammad A. Rashid
Patcharin Krutmuang
Changying Niu
Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions
description The Chinese citrus fruit fly, Bactrocera minax, is an economically important pest of citrus. It exhibits pupal diapause from November to May to combat harsh environmental conditions. Such a long pupal diapause is a barrier for laboratory rearing and development of control strategies against this pest. In the present study, 20-hydroxyecdysone (20E) was used to break pupal diapause of B. minax by topical application. After diapause termination by 20E treated, the pupal ontogenetic processes were observed along the temporal trajectory. The pupal response time to 20E was estimated by detecting the relative expression of 20E responsive genes at different times after 20E-treatment. Results revealed that 20E could effectively terminate the pupal diapause in a dose-dependent manner and significantly shorten the time for 50% adult emergence (Et 50). 20E response genes, including ecr, broad and foxo, were up-regulated within 72h, indicating these genes are involved in pupal metamorphosis and diapause termination processes. Morphological changes showed the pupal metamorphosis began ∼7 days after 20E-treatment at 22 °C. This study does not only pave the way for artificial rearing in the laboratory through manipulating of pupal diapause termination, but also deepens our understanding of the underlying pupal diapause termination mechanism of B. minax.
format Journal
author Zhenzhong Chen
Yongcheng Dong
Yaohui Wang
Awawing A. Andongma
Muhammad A. Rashid
Patcharin Krutmuang
Changying Niu
author_facet Zhenzhong Chen
Yongcheng Dong
Yaohui Wang
Awawing A. Andongma
Muhammad A. Rashid
Patcharin Krutmuang
Changying Niu
author_sort Zhenzhong Chen
title Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions
title_short Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions
title_full Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions
title_fullStr Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions
title_full_unstemmed Pupal diapause termination in Bactrocera minax: An insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions
title_sort pupal diapause termination in bactrocera minax: an insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84976469659&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56358
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