Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine

© 2017 Houghton Trust Ltd. A previous study demonstrated that a recombinant outer membrane protein H (rOmpH)-based intranasal fowl cholera vaccine elicited efficient homologous protection against the Pasteurella multocida strain X-73 (A:1) in chickens. The present study aimed to determine the cross-...

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Main Authors: Thanya Varinrak, Pichayanut Poolperm, Takuo Sawada, Nattawooti Sthitmatee
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/56453
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spelling th-cmuir.6653943832-564532018-09-05T03:55:11Z Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine Thanya Varinrak Pichayanut Poolperm Takuo Sawada Nattawooti Sthitmatee Agricultural and Biological Sciences Immunology and Microbiology Veterinary © 2017 Houghton Trust Ltd. A previous study demonstrated that a recombinant outer membrane protein H (rOmpH)-based intranasal fowl cholera vaccine elicited efficient homologous protection against the Pasteurella multocida strain X-73 (A:1) in chickens. The present study aimed to determine the cross-protectivity against heterologous P. multocida strains. The rOmpH was purified via electroelution and formulated with two kinds of adjuvants. The vaccine formulations in a total volume of 100 µl were 100 µg rOmpH with 3 µg of Escherichia coli enterotoxin B or 10 µg of CpG ODN2007. Chickens were assigned to three experimental groups depending on bacterial strain challenge exposure as well as three control groups. The chickens were immunized intranasally three times at three-week intervals. Challenge exposures were conducted by inoculation with homologous strain X-73 or heterologous strains P-1059 (A:3) or P-1662 (A:4) at four weeks after the final immunization. The specific antibody against rOmpH was produced in vaccinated birds. Sera IgY and secretory IgA antibody titres were significantly increased (P < 0.05) post-immunization. The stimulation index values of the vaccinated groups were significantly different from stimulation index values of the non-vaccinated groups (P < 0.05). Chicken survival rates after exposure to avian P. multocida strains ranged from 70% to 100%. There was no significant difference in protection between two kinds of adjuvants in vaccine formulations. Statistical analysis indicated no significant differences in protection among avian P. multocida strains challenge exposure. We conclude that an in-house rOmpH-based intranasal fowl cholera vaccine produced efficient cross-protectivity against heterologous strains of P. multocida. 2018-09-05T03:26:29Z 2018-09-05T03:26:29Z 2017-09-03 Journal 14653338 03079457 2-s2.0-85019733415 10.1080/03079457.2017.1321105 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019733415&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56453
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Immunology and Microbiology
Veterinary
spellingShingle Agricultural and Biological Sciences
Immunology and Microbiology
Veterinary
Thanya Varinrak
Pichayanut Poolperm
Takuo Sawada
Nattawooti Sthitmatee
Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine
description © 2017 Houghton Trust Ltd. A previous study demonstrated that a recombinant outer membrane protein H (rOmpH)-based intranasal fowl cholera vaccine elicited efficient homologous protection against the Pasteurella multocida strain X-73 (A:1) in chickens. The present study aimed to determine the cross-protectivity against heterologous P. multocida strains. The rOmpH was purified via electroelution and formulated with two kinds of adjuvants. The vaccine formulations in a total volume of 100 µl were 100 µg rOmpH with 3 µg of Escherichia coli enterotoxin B or 10 µg of CpG ODN2007. Chickens were assigned to three experimental groups depending on bacterial strain challenge exposure as well as three control groups. The chickens were immunized intranasally three times at three-week intervals. Challenge exposures were conducted by inoculation with homologous strain X-73 or heterologous strains P-1059 (A:3) or P-1662 (A:4) at four weeks after the final immunization. The specific antibody against rOmpH was produced in vaccinated birds. Sera IgY and secretory IgA antibody titres were significantly increased (P < 0.05) post-immunization. The stimulation index values of the vaccinated groups were significantly different from stimulation index values of the non-vaccinated groups (P < 0.05). Chicken survival rates after exposure to avian P. multocida strains ranged from 70% to 100%. There was no significant difference in protection between two kinds of adjuvants in vaccine formulations. Statistical analysis indicated no significant differences in protection among avian P. multocida strains challenge exposure. We conclude that an in-house rOmpH-based intranasal fowl cholera vaccine produced efficient cross-protectivity against heterologous strains of P. multocida.
format Journal
author Thanya Varinrak
Pichayanut Poolperm
Takuo Sawada
Nattawooti Sthitmatee
author_facet Thanya Varinrak
Pichayanut Poolperm
Takuo Sawada
Nattawooti Sthitmatee
author_sort Thanya Varinrak
title Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine
title_short Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine
title_full Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine
title_fullStr Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine
title_full_unstemmed Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine
title_sort cross-protection conferred by immunization with an romph-based intranasal fowl cholera vaccine
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019733415&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56453
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