Mutations of the β-tubulin gene fragments from carbendazim-resistant isolates of Pestalotiopsis sp. causing strawberry leaf blight in Chiang Mai, Thailand

• Main Authors: Jitparpat Kummanid, Kazuya Akimitsu, Sarunya Nalumpang
• Format: Journal
• Published: 2018
• Subjects: Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85020105754&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/56476
• Institution: Chiang Mai University

© 2017 Blackwell Verlag GmbH Screening of field isolates of Pestalotiopsis sp. from strawberry leaf blight in Thailand identified 56 carbendazim-resistant isolates. Of 56 isolates, 39 highly resistant (HR) phenotypes grew well on PDA amended with carbendazim even at ≥500 mg/L. Isolates with carbendazim-resistant phenotype had a conspicuous mutation at particular sites in the beta-tubulin (β-tubulin) gene sequence. A β-tubulin encoding gene from this pathogen was cloned and sequenced. Analysis of the β-tubulin gene in highly resistant (HR) isolates showed a substitution at codon 79 and 102 from serine (AGT) to lysine (AAA) and valine (GTA) to alanine (GCA), respectively. The detection of such point mutations in the β-tubulin gene allows the rapid screening to detect carbendazim-resistant isolates in the field.