Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity

© 2016, American Association of Pharmaceutical Scientists. The present study aims to investigate the major constituents of the essential oil from Zingiber cassumunar rhizome (EO) and to develop microemulsions with enhanced chemical stability and anti-inflammatory activity of EO. The major constituen...

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Main Authors: Wantida Chaiyana, Songyot Anuchapreeda, Pimporn Leelapornpisid, Rungsinee Phongpradist, Helmut Viernstein, Monika Mueller
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/56521
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-565212018-09-05T03:51:49Z Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity Wantida Chaiyana Songyot Anuchapreeda Pimporn Leelapornpisid Rungsinee Phongpradist Helmut Viernstein Monika Mueller Agricultural and Biological Sciences Pharmacology, Toxicology and Pharmaceutics © 2016, American Association of Pharmaceutical Scientists. The present study aims to investigate the major constituents of the essential oil from Zingiber cassumunar rhizome (EO) and to develop microemulsions with enhanced chemical stability and anti-inflammatory activity of EO. The major constituents of EO were terpinen-4-ol (40.5 ± 6.6%) and sabinene (17.4 ± 1.4%) as determined by gas chromatography–mass spectrometry. These compounds were responsible for the anti-inflammatory activities of EO. Sabinene and terpinen-4-ol significantly reduced nuclear factor-kappa B (NF-kB) expression by 47 ± 5 and 78 ± 8%, respectively (p < 0.001) and significantly reduced the interleukin-6 (IL-6) secretion levels to 64 ± 4% (p < 0.05) and 50 ± 1% (p < 0.001), respectively. EO microemulsions, developed using the system of EO/Tween 20 and propylene glycol (2:1)/water, showed the internal droplet size in the range of 211.5 ± 63.3 to 366.7 ± 77.8 nm. Both EO and EO microemulsions were shown to be safe for human use since there was no apparent toxic effect on human peripheral blood mononuclear cells. Interestingly, EO microemulsion could significantly protect sabinene from the evaporation after heating–cooling stability test, which leads to a good stability and high efficacy. Moreover, EO microemulsions significantly enhanced the anti-inflammatory effect comparing to the native EO. Therefore, microemulsions were attractive delivery system for natural anti-inflammatory compounds since they could enhance both efficacy and stability of EO. 2018-09-05T03:27:11Z 2018-09-05T03:27:11Z 2017-05-01 Journal 15309932 2-s2.0-85028256795 10.1208/s12249-016-0603-2 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028256795&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56521
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Agricultural and Biological Sciences
Pharmacology, Toxicology and Pharmaceutics
Wantida Chaiyana
Songyot Anuchapreeda
Pimporn Leelapornpisid
Rungsinee Phongpradist
Helmut Viernstein
Monika Mueller
Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity
description © 2016, American Association of Pharmaceutical Scientists. The present study aims to investigate the major constituents of the essential oil from Zingiber cassumunar rhizome (EO) and to develop microemulsions with enhanced chemical stability and anti-inflammatory activity of EO. The major constituents of EO were terpinen-4-ol (40.5 ± 6.6%) and sabinene (17.4 ± 1.4%) as determined by gas chromatography–mass spectrometry. These compounds were responsible for the anti-inflammatory activities of EO. Sabinene and terpinen-4-ol significantly reduced nuclear factor-kappa B (NF-kB) expression by 47 ± 5 and 78 ± 8%, respectively (p < 0.001) and significantly reduced the interleukin-6 (IL-6) secretion levels to 64 ± 4% (p < 0.05) and 50 ± 1% (p < 0.001), respectively. EO microemulsions, developed using the system of EO/Tween 20 and propylene glycol (2:1)/water, showed the internal droplet size in the range of 211.5 ± 63.3 to 366.7 ± 77.8 nm. Both EO and EO microemulsions were shown to be safe for human use since there was no apparent toxic effect on human peripheral blood mononuclear cells. Interestingly, EO microemulsion could significantly protect sabinene from the evaporation after heating–cooling stability test, which leads to a good stability and high efficacy. Moreover, EO microemulsions significantly enhanced the anti-inflammatory effect comparing to the native EO. Therefore, microemulsions were attractive delivery system for natural anti-inflammatory compounds since they could enhance both efficacy and stability of EO.
format Journal
author Wantida Chaiyana
Songyot Anuchapreeda
Pimporn Leelapornpisid
Rungsinee Phongpradist
Helmut Viernstein
Monika Mueller
author_facet Wantida Chaiyana
Songyot Anuchapreeda
Pimporn Leelapornpisid
Rungsinee Phongpradist
Helmut Viernstein
Monika Mueller
author_sort Wantida Chaiyana
title Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity
title_short Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity
title_full Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity
title_fullStr Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity
title_full_unstemmed Development of Microemulsion Delivery System of Essential Oil from Zingiber cassumunar Roxb. Rhizome for Improvement of Stability and Anti-Inflammatory Activity
title_sort development of microemulsion delivery system of essential oil from zingiber cassumunar roxb. rhizome for improvement of stability and anti-inflammatory activity
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028256795&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56521
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