Rapid detection of myrosinase-producing fungi: A plate method based on opaque barium sulphate formation
A simple and rapid technique to assess the capability of fungi to produce myrosinase is reported. This was carried out by growing the tested fungi in sinigrin-barium agar plates. Strains capable of producing myrosinase were indicated by an opaque barium sulphate zone forming underneath and/or surrou...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2014
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| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0036176933&partnerID=40&md5=0c952f1d518dd627085bba7e57f3480f http://cmuir.cmu.ac.th/handle/6653943832/5661 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | A simple and rapid technique to assess the capability of fungi to produce myrosinase is reported. This was carried out by growing the tested fungi in sinigrin-barium agar plates. Strains capable of producing myrosinase were indicated by an opaque barium sulphate zone forming underneath and/or surrounding their colonies. This simple test has been confirmed by determination of myrosinase activity in liquid culture. In positive isolates, enzyme acitivity was detected in cell-free extracts, not in culture filtrates. In the case of non-myrosinase-producing strains, no opaque zone was observed and the enzyme was not detected either in cell-free extracts or in culture filtrates. |
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