Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells

© 2017 Elsevier Ltd Background and objective Based on our earlier work on the response of periodontal ligament (PDL) cells to mechanical stress by induction of cyclooxygenase expression and production of prostaglandin PGE2that could regulate mineralization of PDL cells, it was hypothesized that PGE2...

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Main Authors: Avirut Truntipakorn, Anupong Makeudom, Thanapat Sastraruji, Prasit Pavasant, Kassara Pattamapun, Suttichai Krisanaprakornkit
Format: Journal
Published: 2018
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spelling th-cmuir.6653943832-566792018-09-05T03:46:27Z Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells Avirut Truntipakorn Anupong Makeudom Thanapat Sastraruji Prasit Pavasant Kassara Pattamapun Suttichai Krisanaprakornkit Biochemistry, Genetics and Molecular Biology Dentistry Medicine © 2017 Elsevier Ltd Background and objective Based on our earlier work on the response of periodontal ligament (PDL) cells to mechanical stress by induction of cyclooxygenase expression and production of prostaglandin PGE2that could regulate mineralization of PDL cells, it was hypothesized that PGE2had potential effects on PDL stemness. In this study, we aimed to investigate clonogenicity, proliferation and expression of certain pluripotent markers, considered to be characteristics of PDL stemness, in response to treatment with exogenously-added PGE2. Material and methods Human PDL cells were cultured and treated with various doses of PGE2, and the aforementioned characteristics of PDL stemness were analyzed. Results The clonogenicity and proliferation were significantly enhanced by PGE2at low concentrations (0.01, 0.1 and 1 ng/ml; P < 0.05), but only the proliferation was significantly diminished by PGE2at a high concentration (100 ng/ml; P < 0.05). Expression of NANOG and OCT4 mRNA and protein was increased by PGE2treatment at 0.1 and 1 ng/ml. Consistently, expression of stage-specific embryonic antigen 4, a putative stem cell marker, was significantly augmented by PGE2treatment at 1 ng/ml (P < 0.05). Conclusion Our findings suggest that although a high dose of PGE2(100 ng/ml) inhibits proliferation of PDL cells, PGE2at low doses appears to play a role in the maintenance of PDL stemness. 2018-09-05T03:28:48Z 2018-09-05T03:28:48Z 2017-11-01 Journal 18791506 00039969 2-s2.0-85026384092 10.1016/j.archoralbio.2017.07.017 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026384092&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56679
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Dentistry
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Dentistry
Medicine
Avirut Truntipakorn
Anupong Makeudom
Thanapat Sastraruji
Prasit Pavasant
Kassara Pattamapun
Suttichai Krisanaprakornkit
Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells
description © 2017 Elsevier Ltd Background and objective Based on our earlier work on the response of periodontal ligament (PDL) cells to mechanical stress by induction of cyclooxygenase expression and production of prostaglandin PGE2that could regulate mineralization of PDL cells, it was hypothesized that PGE2had potential effects on PDL stemness. In this study, we aimed to investigate clonogenicity, proliferation and expression of certain pluripotent markers, considered to be characteristics of PDL stemness, in response to treatment with exogenously-added PGE2. Material and methods Human PDL cells were cultured and treated with various doses of PGE2, and the aforementioned characteristics of PDL stemness were analyzed. Results The clonogenicity and proliferation were significantly enhanced by PGE2at low concentrations (0.01, 0.1 and 1 ng/ml; P < 0.05), but only the proliferation was significantly diminished by PGE2at a high concentration (100 ng/ml; P < 0.05). Expression of NANOG and OCT4 mRNA and protein was increased by PGE2treatment at 0.1 and 1 ng/ml. Consistently, expression of stage-specific embryonic antigen 4, a putative stem cell marker, was significantly augmented by PGE2treatment at 1 ng/ml (P < 0.05). Conclusion Our findings suggest that although a high dose of PGE2(100 ng/ml) inhibits proliferation of PDL cells, PGE2at low doses appears to play a role in the maintenance of PDL stemness.
format Journal
author Avirut Truntipakorn
Anupong Makeudom
Thanapat Sastraruji
Prasit Pavasant
Kassara Pattamapun
Suttichai Krisanaprakornkit
author_facet Avirut Truntipakorn
Anupong Makeudom
Thanapat Sastraruji
Prasit Pavasant
Kassara Pattamapun
Suttichai Krisanaprakornkit
author_sort Avirut Truntipakorn
title Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells
title_short Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells
title_full Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells
title_fullStr Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells
title_full_unstemmed Effects of prostaglandin E<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells
title_sort effects of prostaglandin e<inf>2</inf>on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026384092&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56679
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