Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats
© 2017 The Author(s). Both Type 2 diabetes mellitus (T2DM) and estrogen deprivation have been shown to be associated with the development of cardiovascular disease and adverse cardiac remodeling. However, the role of estrogen deprivation on adverse cardiac remodeling in nonobese T2DM rats has not be...
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th-cmuir.6653943832-566832018-09-05T03:28:54Z Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats Nattayaporn Apaijai Narattaphol Charoenphandhu Jitjiroj Ittichaichareon Panan Suntornsaratoon Nateetip Krishnamra Ratchaneevan Aeimlapa Siriporn C. Chattipakorn Nipon Chattipakorn Biochemistry, Genetics and Molecular Biology © 2017 The Author(s). Both Type 2 diabetes mellitus (T2DM) and estrogen deprivation have been shown to be associated with the development of cardiovascular disease and adverse cardiac remodeling. However, the role of estrogen deprivation on adverse cardiac remodeling in nonobese T2DM rats has not been clearly elucidated. We hypothesized that estrogen-deprivation aggravates adverse cardiac remodeling in Goto–Kakizaki (GK) rats. Wild-type (WT) and GK rats at the age of 9 months old were divided into two subgroups to have either a sham operation (WTS, GKS) or a bilateral ovariectomy (WTO, GKO) (n = 6/subgroup). Four months after the operation, the rats were killed, and the heart was excised rapidly. Metabolic parameters, cardiomyocytes hypertrophy, cardiac fibrosis, and biochemical parameters were determined. GK rats had hyperglycemia with hypoinsulinemia, and estrogen deprivation did not increase the severity of T2DM. Cardiac hypertrophy, cardiac oxidative stress, and phosphor-antinuclear factor ?B were higher in WTO and GKS rats than WTS rats, and they markedly increased in GKO rats compared with GKS rats. Furthermore, cardiac fibrosis, transforming growth factor-?, Bax, phosphor-p38, and peroxisome proliferator- activated receptor ? coactivator-1? expression were increased in GKS and GKO rats compared with the lean rats. However, mitochondrial dynamics proteins including dynamin-related protein 1 and mitofusin-2 were not altered by T2DM and estrogen deprivation. Although estrogen deprivation did not aggravate T2DM in GK rats, it increased the severity of cardiac hypertrophy by provoking cardiac inflammation and oxidative stress in nonobese GK rats. 2018-09-05T03:28:54Z 2018-09-05T03:28:54Z 2017-10-31 Journal 15734935 01448463 2-s2.0-85033799058 10.1042/BSR20170886 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85033799058&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56683 |
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Biochemistry, Genetics and Molecular Biology Nattayaporn Apaijai Narattaphol Charoenphandhu Jitjiroj Ittichaichareon Panan Suntornsaratoon Nateetip Krishnamra Ratchaneevan Aeimlapa Siriporn C. Chattipakorn Nipon Chattipakorn Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats |
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© 2017 The Author(s). Both Type 2 diabetes mellitus (T2DM) and estrogen deprivation have been shown to be associated with the development of cardiovascular disease and adverse cardiac remodeling. However, the role of estrogen deprivation on adverse cardiac remodeling in nonobese T2DM rats has not been clearly elucidated. We hypothesized that estrogen-deprivation aggravates adverse cardiac remodeling in Goto–Kakizaki (GK) rats. Wild-type (WT) and GK rats at the age of 9 months old were divided into two subgroups to have either a sham operation (WTS, GKS) or a bilateral ovariectomy (WTO, GKO) (n = 6/subgroup). Four months after the operation, the rats were killed, and the heart was excised rapidly. Metabolic parameters, cardiomyocytes hypertrophy, cardiac fibrosis, and biochemical parameters were determined. GK rats had hyperglycemia with hypoinsulinemia, and estrogen deprivation did not increase the severity of T2DM. Cardiac hypertrophy, cardiac oxidative stress, and phosphor-antinuclear factor ?B were higher in WTO and GKS rats than WTS rats, and they markedly increased in GKO rats compared with GKS rats. Furthermore, cardiac fibrosis, transforming growth factor-?, Bax, phosphor-p38, and peroxisome proliferator- activated receptor ? coactivator-1? expression were increased in GKS and GKO rats compared with the lean rats. However, mitochondrial dynamics proteins including dynamin-related protein 1 and mitofusin-2 were not altered by T2DM and estrogen deprivation. Although estrogen deprivation did not aggravate T2DM in GK rats, it increased the severity of cardiac hypertrophy by provoking cardiac inflammation and oxidative stress in nonobese GK rats. |
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Nattayaporn Apaijai Narattaphol Charoenphandhu Jitjiroj Ittichaichareon Panan Suntornsaratoon Nateetip Krishnamra Ratchaneevan Aeimlapa Siriporn C. Chattipakorn Nipon Chattipakorn |
author_facet |
Nattayaporn Apaijai Narattaphol Charoenphandhu Jitjiroj Ittichaichareon Panan Suntornsaratoon Nateetip Krishnamra Ratchaneevan Aeimlapa Siriporn C. Chattipakorn Nipon Chattipakorn |
author_sort |
Nattayaporn Apaijai |
title |
Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats |
title_short |
Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats |
title_full |
Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats |
title_fullStr |
Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats |
title_full_unstemmed |
Estrogen deprivation aggravates cardiac hypertrophy in nonobese Type 2 diabetic Goto–Kakizaki (GK) rats |
title_sort |
estrogen deprivation aggravates cardiac hypertrophy in nonobese type 2 diabetic goto–kakizaki (gk) rats |
publishDate |
2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85033799058&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56683 |
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1681424737857699840 |