Diversity and plant growth promoting activities of actinomycetes from mangroves

© 2017, Chiang Mai University. All rights reserved. Four hundred forty eight actinomycetes were isolated from water and sediment samples of mangrove areas at Andaman Coastal Research Station for Development of Kasetsart University Research and Development Institute, Ranong province, Thailand. Accord...

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Main Authors: Paweena Suksaard, Wasu Pathom-aree, Kannika Duangmal
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/56696
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spelling th-cmuir.6653943832-566962018-09-05T03:52:52Z Diversity and plant growth promoting activities of actinomycetes from mangroves Paweena Suksaard Wasu Pathom-aree Kannika Duangmal Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2017, Chiang Mai University. All rights reserved. Four hundred forty eight actinomycetes were isolated from water and sediment samples of mangrove areas at Andaman Coastal Research Station for Development of Kasetsart University Research and Development Institute, Ranong province, Thailand. According to the morphological characteristics and occurrence of diaminopimelic acid (DAP) isomeric form in the cell hydrolysates, 318 isolates of actinomycetes were clustered as members of streptomycete and 130 isolates were clustered as non-streptomycete type. These 448 isolates could be separated into 8 spore-colour groups and 16S rRNA gene sequences of 146 representative isolates, which had different morphologies from each colour group, were analyzed. They were classified into 14 genera: Actinomadura, Actinoplanes, Gordonia, Isoptericola, Jiangella, Microbispora, Micromonospora, Nocardia, Nocardiopsis, Nonomuraea, Pseudonocardia, Saccharopolyspora, Streptomyces and Streptosporangium. Streptomyces was most prevalent genus with 65.1 % occurrence frequency followed by Micromonospora (6.8 %), Nonomuraea (6.2 %) and Saccharopolyspora (6.2 %). Assessment of plant growth promoting activities of the 448 isolates exhibited that 88.4 %, 50.9 % and 38.8 % of them possessed indole-3-acetic acid (IAA), siderophores and phosphate solubilizing activities, respectively. Fifty potent isolates for plant growth promoting activities were further screened for their antagonistic potential against rice pathogenic bacteria (Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola) and aminocyclopropane carboxylate (ACC) deaminase production. Fourteen isolates inhibited both pathogens and 11 isolates exhibited ACC-deaminase activity. Isolates 1SM5-02, 2SH3-07, 3SH5-05 and 3WH5-01 exhibited high plant growth promoting activities even up to 300 mM of NaCl stress. Their 16S rRNA gene sequences were closely related to Nocardiopsis yanglingensis A18T(98.85 %), Streptomyces jiujiangensis JXJ 0074T(99.35 %), Streptomyces psammoticus NBRC 13971T(99.51 %) and Pseudonocardia oroxyli D10T(99.78 %), respectively. The four isolates significantly enhanced shoot and root weight and root length of rice seedling under non-saline and up to 200 mM NaCl conditions. This is the first report on diversity and plant growth promoting potentials of actinomycetes in mangrove from Ranong province, Thailand. 2018-09-05T03:29:00Z 2018-09-05T03:29:00Z 2017-10-01 Journal 01252526 2-s2.0-85030671589 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030671589&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56696
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Paweena Suksaard
Wasu Pathom-aree
Kannika Duangmal
Diversity and plant growth promoting activities of actinomycetes from mangroves
description © 2017, Chiang Mai University. All rights reserved. Four hundred forty eight actinomycetes were isolated from water and sediment samples of mangrove areas at Andaman Coastal Research Station for Development of Kasetsart University Research and Development Institute, Ranong province, Thailand. According to the morphological characteristics and occurrence of diaminopimelic acid (DAP) isomeric form in the cell hydrolysates, 318 isolates of actinomycetes were clustered as members of streptomycete and 130 isolates were clustered as non-streptomycete type. These 448 isolates could be separated into 8 spore-colour groups and 16S rRNA gene sequences of 146 representative isolates, which had different morphologies from each colour group, were analyzed. They were classified into 14 genera: Actinomadura, Actinoplanes, Gordonia, Isoptericola, Jiangella, Microbispora, Micromonospora, Nocardia, Nocardiopsis, Nonomuraea, Pseudonocardia, Saccharopolyspora, Streptomyces and Streptosporangium. Streptomyces was most prevalent genus with 65.1 % occurrence frequency followed by Micromonospora (6.8 %), Nonomuraea (6.2 %) and Saccharopolyspora (6.2 %). Assessment of plant growth promoting activities of the 448 isolates exhibited that 88.4 %, 50.9 % and 38.8 % of them possessed indole-3-acetic acid (IAA), siderophores and phosphate solubilizing activities, respectively. Fifty potent isolates for plant growth promoting activities were further screened for their antagonistic potential against rice pathogenic bacteria (Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola) and aminocyclopropane carboxylate (ACC) deaminase production. Fourteen isolates inhibited both pathogens and 11 isolates exhibited ACC-deaminase activity. Isolates 1SM5-02, 2SH3-07, 3SH5-05 and 3WH5-01 exhibited high plant growth promoting activities even up to 300 mM of NaCl stress. Their 16S rRNA gene sequences were closely related to Nocardiopsis yanglingensis A18T(98.85 %), Streptomyces jiujiangensis JXJ 0074T(99.35 %), Streptomyces psammoticus NBRC 13971T(99.51 %) and Pseudonocardia oroxyli D10T(99.78 %), respectively. The four isolates significantly enhanced shoot and root weight and root length of rice seedling under non-saline and up to 200 mM NaCl conditions. This is the first report on diversity and plant growth promoting potentials of actinomycetes in mangrove from Ranong province, Thailand.
format Journal
author Paweena Suksaard
Wasu Pathom-aree
Kannika Duangmal
author_facet Paweena Suksaard
Wasu Pathom-aree
Kannika Duangmal
author_sort Paweena Suksaard
title Diversity and plant growth promoting activities of actinomycetes from mangroves
title_short Diversity and plant growth promoting activities of actinomycetes from mangroves
title_full Diversity and plant growth promoting activities of actinomycetes from mangroves
title_fullStr Diversity and plant growth promoting activities of actinomycetes from mangroves
title_full_unstemmed Diversity and plant growth promoting activities of actinomycetes from mangroves
title_sort diversity and plant growth promoting activities of actinomycetes from mangroves
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030671589&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56696
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