Electrochemical Detection of Human Interleukin-15 using a Graphene Oxide-Modified Screen-Printed Carbon Electrode

• Main Authors: Poachanee Norfun, Nuttee Suree, Nawee Kungwan, Winita Punyodom, Jaroon Jakmunee, Kontad Ounnunkad
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85021335603&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/56759
• Institution: Chiang Mai University

© 2017 Taylor & Francis. Biofunctionalizing a simple and disposable graphene oxide-modified screen-printed carbon electrode with anti-interleukin-15 antibodies has been successfully demonstrated for the first time for the label-free electrochemical detection of interleukin-15, a biomarker of early HIV infection. To improve the electrochemical reactivity and introduce carboxylic groups on the surface of screen-printed carbon electrode, high-quality graphene oxide was used for the modification of screen-printed carbon electrode. With simple modification of the screen-printed carbon electrode, the device exhibited satisfactory sensitivity, selectivity, stability, reproducibility, and regenerability. The immunosensor provided a detection limit of 3.51 ng mL−1and a sensitivity of 0.5655 µA cm−2mL ng−1. The simply constructed immunosensor thus rendered promising device for immunoreactions on the surface of the electrode.