Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff
© 2017, Chiang Mai University. All rights reserved. The phytochemical screening of the leaves of Graptophyllum pictum (L.) Griff. was carried out using the standard procedures which revealed the presence of flavonoids, steroids, tannins, coumarins, saponins, anthraquinones, phenolics and sugars. In...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010767485&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56833 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-56833 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-568332018-09-05T03:53:57Z Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff Nadechanok Jiangseubchatveera Saisunee Liawruangrath Aphiwat Teerawutgulrag Dammrong Santiarworn Stephen G. Pyne Boonsom Liawruangrath Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2017, Chiang Mai University. All rights reserved. The phytochemical screening of the leaves of Graptophyllum pictum (L.) Griff. was carried out using the standard procedures which revealed the presence of flavonoids, steroids, tannins, coumarins, saponins, anthraquinones, phenolics and sugars. In vitro antioxidant activity, total phenolic and total flavonoid contents of four different fractions from the ethanolic leave extract of G. pictum (L.) Griff. were determined using spectrophotometric methods. The ethyl acetate fraction showed the highest total phenolic content (102.57 ± 0.19 mg gallic acid equivalent/g) and the hexane fraction contained the highest flavonoid (28.21 ± 0.04 mg quercetin equivalent/g). The ethyl acetate fraction also exhibited the highest antioxidant capacities in the DPPH radical scavenging assay with the IC50value of 0.78 ± 0.01 mg/mL and was found 69.19 ± 0.73 mg trolox equivalent capacity/g and 48.04 ± 0.49 mg ascorbic acid equivalent capacity/g when investigating by the ABTS radical scavenging assay. Moreover, the hexane, the ethyl acetate and the aqueous fractions exhibited significant cytotoxicity against MCF-7 cell lines with IC50values of 38.66, 26.01 and 20.41 μg/mL, respectively. All fractions were non-cytotoxic against Vero cells. The results of all experiments suggest that the leaves of G. pictum (L.) Griff. can be a natural candidate for rich source of antioxidants for further chemical investigation. 2018-09-05T03:30:50Z 2018-09-05T03:30:50Z 2017-01-01 Journal 01252526 2-s2.0-85010767485 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010767485&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56833 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
topic |
Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy Nadechanok Jiangseubchatveera Saisunee Liawruangrath Aphiwat Teerawutgulrag Dammrong Santiarworn Stephen G. Pyne Boonsom Liawruangrath Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff |
description |
© 2017, Chiang Mai University. All rights reserved. The phytochemical screening of the leaves of Graptophyllum pictum (L.) Griff. was carried out using the standard procedures which revealed the presence of flavonoids, steroids, tannins, coumarins, saponins, anthraquinones, phenolics and sugars. In vitro antioxidant activity, total phenolic and total flavonoid contents of four different fractions from the ethanolic leave extract of G. pictum (L.) Griff. were determined using spectrophotometric methods. The ethyl acetate fraction showed the highest total phenolic content (102.57 ± 0.19 mg gallic acid equivalent/g) and the hexane fraction contained the highest flavonoid (28.21 ± 0.04 mg quercetin equivalent/g). The ethyl acetate fraction also exhibited the highest antioxidant capacities in the DPPH radical scavenging assay with the IC50value of 0.78 ± 0.01 mg/mL and was found 69.19 ± 0.73 mg trolox equivalent capacity/g and 48.04 ± 0.49 mg ascorbic acid equivalent capacity/g when investigating by the ABTS radical scavenging assay. Moreover, the hexane, the ethyl acetate and the aqueous fractions exhibited significant cytotoxicity against MCF-7 cell lines with IC50values of 38.66, 26.01 and 20.41 μg/mL, respectively. All fractions were non-cytotoxic against Vero cells. The results of all experiments suggest that the leaves of G. pictum (L.) Griff. can be a natural candidate for rich source of antioxidants for further chemical investigation. |
format |
Journal |
author |
Nadechanok Jiangseubchatveera Saisunee Liawruangrath Aphiwat Teerawutgulrag Dammrong Santiarworn Stephen G. Pyne Boonsom Liawruangrath |
author_facet |
Nadechanok Jiangseubchatveera Saisunee Liawruangrath Aphiwat Teerawutgulrag Dammrong Santiarworn Stephen G. Pyne Boonsom Liawruangrath |
author_sort |
Nadechanok Jiangseubchatveera |
title |
Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff |
title_short |
Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff |
title_full |
Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff |
title_fullStr |
Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff |
title_full_unstemmed |
Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff |
title_sort |
phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of graptophyllum pictum (l.) griff |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010767485&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56833 |
_version_ |
1681424765882990592 |