Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production
© FEMS 2016. All rights reserved. Stress-activated MAPK pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidativ...
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th-cmuir.6653943832-568372018-09-05T03:41:49Z Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production Panjaphorn Nimmanee Emily W.T. Tam Patrick C.Y. Woo Pramote Vanittanakom Nongnuch Vanittanakom Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © FEMS 2016. All rights reserved. Stress-activated MAPK pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in asexual development, yeast cell generation in vitro and survival inside macrophage cell lines. In this study, the role of the T. marneffei sakA gene on the nitrosative stress response and the regulation of gene expression were investigated. The susceptibility of the sakA mutant to NaNO2was investigated using drop dilution assay and the expression of genes of interest were determined by RT-PCR, comparing them to the wild-type and complemented strains. The results demonstrated that the T. marneffei sakA gene played a role in the stress response to NaNO2, the expression of genes functioning in conidial development (brlA, abaA and wetA) and red pigment biosynthesis (pks3, rp1, rp2 and rp3). These findings suggest that T. marneffei sakA is broadly involved in a wide variety of cell activities, including stress response, cell morphogenesis, asexual development and pigmentation. 2018-09-05T03:30:56Z 2018-09-05T03:30:56Z 2017-01-01 Journal 15746968 03781097 2-s2.0-85019667707 10.1093/femsle/fnw292 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019667707&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56837 |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Panjaphorn Nimmanee Emily W.T. Tam Patrick C.Y. Woo Pramote Vanittanakom Nongnuch Vanittanakom Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production |
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© FEMS 2016. All rights reserved. Stress-activated MAPK pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in asexual development, yeast cell generation in vitro and survival inside macrophage cell lines. In this study, the role of the T. marneffei sakA gene on the nitrosative stress response and the regulation of gene expression were investigated. The susceptibility of the sakA mutant to NaNO2was investigated using drop dilution assay and the expression of genes of interest were determined by RT-PCR, comparing them to the wild-type and complemented strains. The results demonstrated that the T. marneffei sakA gene played a role in the stress response to NaNO2, the expression of genes functioning in conidial development (brlA, abaA and wetA) and red pigment biosynthesis (pks3, rp1, rp2 and rp3). These findings suggest that T. marneffei sakA is broadly involved in a wide variety of cell activities, including stress response, cell morphogenesis, asexual development and pigmentation. |
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Panjaphorn Nimmanee Emily W.T. Tam Patrick C.Y. Woo Pramote Vanittanakom Nongnuch Vanittanakom |
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Panjaphorn Nimmanee Emily W.T. Tam Patrick C.Y. Woo Pramote Vanittanakom Nongnuch Vanittanakom |
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Panjaphorn Nimmanee |
title |
Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production |
title_short |
Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production |
title_full |
Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production |
title_fullStr |
Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production |
title_full_unstemmed |
Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production |
title_sort |
role of the talaromyces marneffei (penicillium marneffei) saka gene in nitrosative stress response, conidiation and red pigment production |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019667707&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56837 |
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