Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme

© 2017, Chiang Mai University. All rights reserved. The intracellular pyruvate decarboxylase enzyme (PDC, EC 4.1.1.1) extract from Candida tropicalis TISTR 5350 was compared by two different purification methods using ammonium sulphate and acetone precipitation. The total volumetric PDC activity and...

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Main Authors: Julaluk Tangtua, Charin Techapun, Ronachai Pratanaphon, Ampin Kuntiya, Vorapat Sanguanchaipaiwong, Thanongsak Chaiyaso, Prasert Hanmoungjai, Phisit Seesuriyachan, Nopphorn Leksawasdi, Noppol Leksawasdi
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/56845
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spelling th-cmuir.6653943832-568452018-09-05T03:54:08Z Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme Julaluk Tangtua Charin Techapun Ronachai Pratanaphon Ampin Kuntiya Vorapat Sanguanchaipaiwong Thanongsak Chaiyaso Prasert Hanmoungjai Phisit Seesuriyachan Nopphorn Leksawasdi Noppol Leksawasdi Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2017, Chiang Mai University. All rights reserved. The intracellular pyruvate decarboxylase enzyme (PDC, EC 4.1.1.1) extract from Candida tropicalis TISTR 5350 was compared by two different purification methods using ammonium sulphate and acetone precipitation. The total volumetric PDC activity and percentage recovery (yield) of precipitated PDC based on 50% (v/v) cold acetone were significantly higher (1.13 ± 0.02 U/ml and 98.27 ± 2.98 %, respectively) than any other concentration levels of acetone used. Furthermore, all concentration levels of cold acetone also yielded a much higher specific PDC activity than the precipitate obtained using the 40 to 60% (w/v) ammonium sulphate saturation (0.75 ± 0.08 U/mg protein). The precipitated enzyme in buffer solutions from the 50% (v/v) acetone was subsequently freeze dried. Freeze drying of the precipitated PDC by cold acetone resulted in the specific PDC activity of 1.57 ± 0.02 U/mg protein and differed statistically (p ≤ 0.05) from the crude enzyme extract (control). 2018-09-05T03:31:02Z 2018-09-05T03:31:02Z 2017-01-01 Journal 01252526 2-s2.0-85010739408 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010739408&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56845
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Julaluk Tangtua
Charin Techapun
Ronachai Pratanaphon
Ampin Kuntiya
Vorapat Sanguanchaipaiwong
Thanongsak Chaiyaso
Prasert Hanmoungjai
Phisit Seesuriyachan
Nopphorn Leksawasdi
Noppol Leksawasdi
Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
description © 2017, Chiang Mai University. All rights reserved. The intracellular pyruvate decarboxylase enzyme (PDC, EC 4.1.1.1) extract from Candida tropicalis TISTR 5350 was compared by two different purification methods using ammonium sulphate and acetone precipitation. The total volumetric PDC activity and percentage recovery (yield) of precipitated PDC based on 50% (v/v) cold acetone were significantly higher (1.13 ± 0.02 U/ml and 98.27 ± 2.98 %, respectively) than any other concentration levels of acetone used. Furthermore, all concentration levels of cold acetone also yielded a much higher specific PDC activity than the precipitate obtained using the 40 to 60% (w/v) ammonium sulphate saturation (0.75 ± 0.08 U/mg protein). The precipitated enzyme in buffer solutions from the 50% (v/v) acetone was subsequently freeze dried. Freeze drying of the precipitated PDC by cold acetone resulted in the specific PDC activity of 1.57 ± 0.02 U/mg protein and differed statistically (p ≤ 0.05) from the crude enzyme extract (control).
format Journal
author Julaluk Tangtua
Charin Techapun
Ronachai Pratanaphon
Ampin Kuntiya
Vorapat Sanguanchaipaiwong
Thanongsak Chaiyaso
Prasert Hanmoungjai
Phisit Seesuriyachan
Nopphorn Leksawasdi
Noppol Leksawasdi
author_facet Julaluk Tangtua
Charin Techapun
Ronachai Pratanaphon
Ampin Kuntiya
Vorapat Sanguanchaipaiwong
Thanongsak Chaiyaso
Prasert Hanmoungjai
Phisit Seesuriyachan
Nopphorn Leksawasdi
Noppol Leksawasdi
author_sort Julaluk Tangtua
title Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
title_short Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
title_full Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
title_fullStr Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
title_full_unstemmed Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
title_sort partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010739408&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56845
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