Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells

© 2017 Elsevier B.V. Herein, we have developed a targeted imaging nanoprobe for visualizing EpCAM-positive cell lines, especially the hepatocellular carcinoma cell line (HepG2). Mesoporous silica-coated magnetic nanoparticles (mSiO2-MNPs) were first synthesized and then conjugated with EpCAM-specifi...

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Main Authors: Chalermchai Pilapong, Sangsirin Siriwongnanon, Yanee Keereeta
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/57370
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-573702018-09-05T03:53:52Z Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells Chalermchai Pilapong Sangsirin Siriwongnanon Yanee Keereeta Engineering Materials Science Physics and Astronomy © 2017 Elsevier B.V. Herein, we have developed a targeted imaging nanoprobe for visualizing EpCAM-positive cell lines, especially the hepatocellular carcinoma cell line (HepG2). Mesoporous silica-coated magnetic nanoparticles (mSiO2-MNPs) were first synthesized and then conjugated with EpCAM-specific aptamer (EpCAM aptamer) and fluorophore using appropriate bioconjugation routes. The EpCAM nanoprobe is utilized for multimodal imaging including MRI, US, and fluorescence with good physicochemical properties. The nanoprobe showed no toxicity toward either cancer cells (HepG2) or normal cells (PBMC), and demonstrated high cellular uptake in EpCAM+cancer cells, including HepG2, by endocytosis pathway. Apparently, polyvalent EpCAM nanoprobe-EpCAM receptor interaction is required to facilitate internalization across the cellular membrane. Because of high cellular uptake and imaging capability of the nanoprobe, it can be used to efficiently visualize the HepG2 cell using such imaging modalities. The study on the 3D spheroid model clearly confirms that EpCAM nanoprobe has the capability to penetrate into the tumor spheroid while EpCAM aptamer lacks the ability to penetrate into the spheroid on its own. Therefore, EpCAM nanoprobe can be used for targeted EpCAM+cancer cell imaging with the potential for improved tumor penetration. This development might lead to be a new biosensing technology, especially for imaging based in vivo detection. 2018-09-05T03:39:32Z 2018-09-05T03:39:32Z 2017-01-01 Journal 09254005 2-s2.0-85011876472 10.1016/j.snb.2017.02.012 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85011876472&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/57370
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Engineering
Materials Science
Physics and Astronomy
spellingShingle Engineering
Materials Science
Physics and Astronomy
Chalermchai Pilapong
Sangsirin Siriwongnanon
Yanee Keereeta
Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells
description © 2017 Elsevier B.V. Herein, we have developed a targeted imaging nanoprobe for visualizing EpCAM-positive cell lines, especially the hepatocellular carcinoma cell line (HepG2). Mesoporous silica-coated magnetic nanoparticles (mSiO2-MNPs) were first synthesized and then conjugated with EpCAM-specific aptamer (EpCAM aptamer) and fluorophore using appropriate bioconjugation routes. The EpCAM nanoprobe is utilized for multimodal imaging including MRI, US, and fluorescence with good physicochemical properties. The nanoprobe showed no toxicity toward either cancer cells (HepG2) or normal cells (PBMC), and demonstrated high cellular uptake in EpCAM+cancer cells, including HepG2, by endocytosis pathway. Apparently, polyvalent EpCAM nanoprobe-EpCAM receptor interaction is required to facilitate internalization across the cellular membrane. Because of high cellular uptake and imaging capability of the nanoprobe, it can be used to efficiently visualize the HepG2 cell using such imaging modalities. The study on the 3D spheroid model clearly confirms that EpCAM nanoprobe has the capability to penetrate into the tumor spheroid while EpCAM aptamer lacks the ability to penetrate into the spheroid on its own. Therefore, EpCAM nanoprobe can be used for targeted EpCAM+cancer cell imaging with the potential for improved tumor penetration. This development might lead to be a new biosensing technology, especially for imaging based in vivo detection.
format Journal
author Chalermchai Pilapong
Sangsirin Siriwongnanon
Yanee Keereeta
author_facet Chalermchai Pilapong
Sangsirin Siriwongnanon
Yanee Keereeta
author_sort Chalermchai Pilapong
title Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells
title_short Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells
title_full Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells
title_fullStr Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells
title_full_unstemmed Development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells
title_sort development of targeted multimodal imaging agent in ionizing radiation-free approach for visualizing hepatocellular carcinoma cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85011876472&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/57370
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