Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic conditions

A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3 at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+ to the NH 4NO3 medium markedly promoted the utilization of NH 4+ and NO3-. At 50°C, of total nitrogen originally provided, 24% w...

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Bibliographic Details
Main Authors: Takenaka S., Zhou Q., Kuntiya A., Seesuriyachan P., Murakami S., Aoki K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-33847318870&partnerID=40&md5=c5e7c0e506932016c227fa048af25f91
http://cmuir.cmu.ac.th/handle/6653943832/575
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Institution: Chiang Mai University
Language: English
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Summary:A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3 at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+ to the NH 4NO3 medium markedly promoted the utilization of NH 4+ and NO3-. At 50°C, of total nitrogen originally provided, 24% was taken up into the cells and 20% remained in the culture supernatant. Residual nitrogen (56%) was probably removed into the atmosphere. The cell extracts contained enzymes involved in denitrification. GC-MS demonstrated that NH415NO3 had been converted to 15N2O. These results indicate that the strain has denitrification ability under aerobic condition. © 2007 Springer Science+Business Media B.V.