Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic conditions
A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3 at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+ to the NH 4NO3 medium markedly promoted the utilization of NH 4+ and NO3-. At 50°C, of total nitrogen originally provided, 24% w...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
2014
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Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-33847318870&partnerID=40&md5=c5e7c0e506932016c227fa048af25f91 http://cmuir.cmu.ac.th/handle/6653943832/575 |
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Institution: | Chiang Mai University |
Language: | English |
Summary: | A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3 at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+ to the NH 4NO3 medium markedly promoted the utilization of NH 4+ and NO3-. At 50°C, of total nitrogen originally provided, 24% was taken up into the cells and 20% remained in the culture supernatant. Residual nitrogen (56%) was probably removed into the atmosphere. The cell extracts contained enzymes involved in denitrification. GC-MS demonstrated that NH415NO3 had been converted to 15N2O. These results indicate that the strain has denitrification ability under aerobic condition. © 2007 Springer Science+Business Media B.V. |
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