Extraction and evaluation of pectin methylesterase from Morinda citrifolia
© 2017 Manee Saelee et al. Morinda citrifolia Linn. (Noni) is used in traditional medicine. Pectinmethylesterases (PME) is an enzyme that is responsible for the degradation of pectin. The current study was aimed to extract PME from M. citrifolia (MPME) and compared with commercial PME, and studied t...
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th-cmuir.6653943832-575822018-09-05T03:51:28Z Extraction and evaluation of pectin methylesterase from Morinda citrifolia Manee Saelee Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Chaiyavat Chaiyasut Medicine Pharmacology, Toxicology and Pharmaceutics © 2017 Manee Saelee et al. Morinda citrifolia Linn. (Noni) is used in traditional medicine. Pectinmethylesterases (PME) is an enzyme that is responsible for the degradation of pectin. The current study was aimed to extract PME from M. citrifolia (MPME) and compared with commercial PME, and studied the temperature mediated inactivation of PME. The results revealed that the optimum time, pH, and temperature of MPME was found as 5 min, pH 7, and 30°C, respectively. The pretreatment of M. citrifolia for 5 min at 100°C can significantly prevent the methanol formation. Further detailed study on the purification and characterization of MPME and the influence of pretreatment of M. citrifolia on methanol formation under progress. 2018-09-05T03:46:19Z 2018-09-05T03:46:19Z 2017-11-01 Journal 22313354 2-s2.0-85037051847 10.7324/JAPS.2017.71128 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85037051847&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/57582 |
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Medicine Pharmacology, Toxicology and Pharmaceutics Manee Saelee Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Chaiyavat Chaiyasut Extraction and evaluation of pectin methylesterase from Morinda citrifolia |
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© 2017 Manee Saelee et al. Morinda citrifolia Linn. (Noni) is used in traditional medicine. Pectinmethylesterases (PME) is an enzyme that is responsible for the degradation of pectin. The current study was aimed to extract PME from M. citrifolia (MPME) and compared with commercial PME, and studied the temperature mediated inactivation of PME. The results revealed that the optimum time, pH, and temperature of MPME was found as 5 min, pH 7, and 30°C, respectively. The pretreatment of M. citrifolia for 5 min at 100°C can significantly prevent the methanol formation. Further detailed study on the purification and characterization of MPME and the influence of pretreatment of M. citrifolia on methanol formation under progress. |
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Journal |
author |
Manee Saelee Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Chaiyavat Chaiyasut |
author_facet |
Manee Saelee Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Chaiyavat Chaiyasut |
author_sort |
Manee Saelee |
title |
Extraction and evaluation of pectin methylesterase from Morinda citrifolia |
title_short |
Extraction and evaluation of pectin methylesterase from Morinda citrifolia |
title_full |
Extraction and evaluation of pectin methylesterase from Morinda citrifolia |
title_fullStr |
Extraction and evaluation of pectin methylesterase from Morinda citrifolia |
title_full_unstemmed |
Extraction and evaluation of pectin methylesterase from Morinda citrifolia |
title_sort |
extraction and evaluation of pectin methylesterase from morinda citrifolia |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85037051847&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/57582 |
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