Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes
© 2017 Mingkwan Na Takuathung et al. Psoriasis is a chronic inflammatory and immune-mediated skin disease. The pathogenesis involves T cells activation via the IL-23/Th17 axis. Conventional treatments of psoriasis have adverse events influencing patients' adherence. Wannachawee Recipe (WCR) has...
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th-cmuir.6653943832-578042018-09-05T03:50:02Z Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes Mingkwan Na Takuathung Ariyaphong Wongnoppavich Pornsiri Pitchakarn Ampai Panthong Parirat Khonsung Natthakarn Chiranthanut Noppamas Soonthornchareonnon Seewaboon Sireeratawong Medicine © 2017 Mingkwan Na Takuathung et al. Psoriasis is a chronic inflammatory and immune-mediated skin disease. The pathogenesis involves T cells activation via the IL-23/Th17 axis. Conventional treatments of psoriasis have adverse events influencing patients' adherence. Wannachawee Recipe (WCR) has been effectively used as Thai folk remedy for psoriasis patients; however, preclinical evidence defining how WCR works is still lacking. This study defined mechanisms for its antiproliferation and anti-inflammatory effects in HaCaT cells. The cytotoxicity and antiproliferation results from SRB and CCK-8 assays showed that WCR inhibited the growth and viability of HaCaT cells in a concentration-dependent manner. The distribution of cell cycle phases determined by flow cytometry showed that WCR did not interrupt cell cycle progression. Interestingly, RT-qPCR revealed that WCR significantly decreased the mRNA expression of IL-1β, IL-6, IL-8, IL-17A, IL-22, IL-23, and TNF-α but induced IL-10 expression in TNF-α- and IFN-γ-induced HaCaT cells. At the protein level determined by ELISA, WCR significantly reduced the secretion of IL-17A, IL-22, and IL-23. The WCR at low concentrations was proved to possess anti-inflammatory effect without cytotoxicity and it did not interfere with cell cycle of keratinocytes. This is the first study to provide convincing evidence that WCR is a potential candidate for development of effective psoriasis therapies. 2018-09-05T03:50:02Z 2018-09-05T03:50:02Z 2017-01-01 Journal 17414288 1741427X 2-s2.0-85028657082 10.1155/2017/5906539 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028657082&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/57804 |
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Medicine Mingkwan Na Takuathung Ariyaphong Wongnoppavich Pornsiri Pitchakarn Ampai Panthong Parirat Khonsung Natthakarn Chiranthanut Noppamas Soonthornchareonnon Seewaboon Sireeratawong Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes |
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© 2017 Mingkwan Na Takuathung et al. Psoriasis is a chronic inflammatory and immune-mediated skin disease. The pathogenesis involves T cells activation via the IL-23/Th17 axis. Conventional treatments of psoriasis have adverse events influencing patients' adherence. Wannachawee Recipe (WCR) has been effectively used as Thai folk remedy for psoriasis patients; however, preclinical evidence defining how WCR works is still lacking. This study defined mechanisms for its antiproliferation and anti-inflammatory effects in HaCaT cells. The cytotoxicity and antiproliferation results from SRB and CCK-8 assays showed that WCR inhibited the growth and viability of HaCaT cells in a concentration-dependent manner. The distribution of cell cycle phases determined by flow cytometry showed that WCR did not interrupt cell cycle progression. Interestingly, RT-qPCR revealed that WCR significantly decreased the mRNA expression of IL-1β, IL-6, IL-8, IL-17A, IL-22, IL-23, and TNF-α but induced IL-10 expression in TNF-α- and IFN-γ-induced HaCaT cells. At the protein level determined by ELISA, WCR significantly reduced the secretion of IL-17A, IL-22, and IL-23. The WCR at low concentrations was proved to possess anti-inflammatory effect without cytotoxicity and it did not interfere with cell cycle of keratinocytes. This is the first study to provide convincing evidence that WCR is a potential candidate for development of effective psoriasis therapies. |
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Mingkwan Na Takuathung Ariyaphong Wongnoppavich Pornsiri Pitchakarn Ampai Panthong Parirat Khonsung Natthakarn Chiranthanut Noppamas Soonthornchareonnon Seewaboon Sireeratawong |
author_facet |
Mingkwan Na Takuathung Ariyaphong Wongnoppavich Pornsiri Pitchakarn Ampai Panthong Parirat Khonsung Natthakarn Chiranthanut Noppamas Soonthornchareonnon Seewaboon Sireeratawong |
author_sort |
Mingkwan Na Takuathung |
title |
Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes |
title_short |
Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes |
title_full |
Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes |
title_fullStr |
Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes |
title_full_unstemmed |
Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes |
title_sort |
effects of wannachawee recipe with antipsoriatic activity on suppressing inflammatory cytokine production in hacat human keratinocytes |
publishDate |
2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028657082&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/57804 |
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1681424946829459456 |