Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells

© 2017 Ratchadaporn Namsen et al. Leaves of Thunbergia laurifolia (TL) have been reported to have antioxidation, anti-inflammatory, detoxifying, and hepatoprotective effects. However, studies relating to antifibrotic activity have not been reported. Currently, there is no standard treatment for hepa...

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Main Authors: Ratchadaporn Namsen, Noppamas Rojanasthien, Seewaboon Sireeratawong, Piyanuch Rojsanga, Wutigri Nimlamool, Saranyapin Potikanond
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/57807
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spelling th-cmuir.6653943832-578072018-09-05T03:50:03Z Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells Ratchadaporn Namsen Noppamas Rojanasthien Seewaboon Sireeratawong Piyanuch Rojsanga Wutigri Nimlamool Saranyapin Potikanond Medicine © 2017 Ratchadaporn Namsen et al. Leaves of Thunbergia laurifolia (TL) have been reported to have antioxidation, anti-inflammatory, detoxifying, and hepatoprotective effects. However, studies relating to antifibrotic activity have not been reported. Currently, there is no standard treatment for hepatic fibrosis. This study aimed to investigate the antifibrotic activity of TL in human hepatic stellate LX-2 cells. Results from cell viability and cell death assays showed that the extract at high concentrations was toxic to LX-2 cells. TL extract reversed the transformation of LX-2 cells to myofibroblast-like characteristics in response to stimulation by transforming growth factor-beta 1. This action may be associated with the effect of TL in suppressing α-SMA and collagen-I production observed by immunofluorescence study and western blot analysis. Additionally, TL extract significantly decreased MMP-9 activity which is consistent with the reduction of MMP-9, MMP-2, and TIMP-1 gene expression. The effect of TL in suppressing fibrosis may be associated with its ability to inhibit the activation of p38 MAPK and Erk1/2 kinases as examined by western blot analysis. Our study provides convincing evidence that TL possesses antifibrotic activity which may be through the suppression of TGF-β1-mediated production of MMPs, collagen-1, and α-SMA in hepatic stellate cells. 2018-09-05T03:50:03Z 2018-09-05T03:50:03Z 2017-01-01 Journal 17414288 1741427X 2-s2.0-85042681913 10.1155/2017/3508569 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042681913&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/57807
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Ratchadaporn Namsen
Noppamas Rojanasthien
Seewaboon Sireeratawong
Piyanuch Rojsanga
Wutigri Nimlamool
Saranyapin Potikanond
Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells
description © 2017 Ratchadaporn Namsen et al. Leaves of Thunbergia laurifolia (TL) have been reported to have antioxidation, anti-inflammatory, detoxifying, and hepatoprotective effects. However, studies relating to antifibrotic activity have not been reported. Currently, there is no standard treatment for hepatic fibrosis. This study aimed to investigate the antifibrotic activity of TL in human hepatic stellate LX-2 cells. Results from cell viability and cell death assays showed that the extract at high concentrations was toxic to LX-2 cells. TL extract reversed the transformation of LX-2 cells to myofibroblast-like characteristics in response to stimulation by transforming growth factor-beta 1. This action may be associated with the effect of TL in suppressing α-SMA and collagen-I production observed by immunofluorescence study and western blot analysis. Additionally, TL extract significantly decreased MMP-9 activity which is consistent with the reduction of MMP-9, MMP-2, and TIMP-1 gene expression. The effect of TL in suppressing fibrosis may be associated with its ability to inhibit the activation of p38 MAPK and Erk1/2 kinases as examined by western blot analysis. Our study provides convincing evidence that TL possesses antifibrotic activity which may be through the suppression of TGF-β1-mediated production of MMPs, collagen-1, and α-SMA in hepatic stellate cells.
format Journal
author Ratchadaporn Namsen
Noppamas Rojanasthien
Seewaboon Sireeratawong
Piyanuch Rojsanga
Wutigri Nimlamool
Saranyapin Potikanond
author_facet Ratchadaporn Namsen
Noppamas Rojanasthien
Seewaboon Sireeratawong
Piyanuch Rojsanga
Wutigri Nimlamool
Saranyapin Potikanond
author_sort Ratchadaporn Namsen
title Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells
title_short Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells
title_full Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells
title_fullStr Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells
title_full_unstemmed Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells
title_sort thunbergia laurifolia exhibits antifibrotic effects in human hepatic stellate cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042681913&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/57807
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