Development of immunochromatographic assay for the on-site detection of salbutamol

• Main Authors: Khamta Y., Pattarawarapan M., Nangola S., Tayapiwatana C.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-70449730062&partnerID=40&md5=86cec7c83a03bbec57dd4ca171516bd0; http://www.ncbi.nlm.nih.gov/pubmed/19739017; http://cmuir.cmu.ac.th/handle/6653943832/5798
• Institution: Chiang Mai University

Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80ng/mL of salbutamol in PBS. The method can be useful as an on-site screening procedure for detection of salbutamol.