Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi

© 2018 Elsevier B.V. In Southeast Asia, Anopheles lesteri (recently synonymized with An. paraliae) is a competent vector for Plasmodium parasites, but its ability to transmit parasites that cause lymphatic filariasis has yet to be determined. In this study, the susceptibility of An. lesteri and An....

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Main Authors: Watcharatip Dedkhad, Lyric C. Bartholomay, Bruce M. Christensen, Deepak Joshi, Kritsana Taai, Chayanit Hempolchom, Atiporn Saeung
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58000
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-580002018-09-05T04:40:54Z Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi Watcharatip Dedkhad Lyric C. Bartholomay Bruce M. Christensen Deepak Joshi Kritsana Taai Chayanit Hempolchom Atiporn Saeung Agricultural and Biological Sciences Immunology and Microbiology Medicine Veterinary © 2018 Elsevier B.V. In Southeast Asia, Anopheles lesteri (recently synonymized with An. paraliae) is a competent vector for Plasmodium parasites, but its ability to transmit parasites that cause lymphatic filariasis has yet to be determined. In this study, the susceptibility of An. lesteri and An. paraliae to Brugia malayi parasites was determined by comparing with the control mosquito, Aedes togoi. We found that the infection prevalence per infected mosquito in An. paraliae was significantly lower than that in Ae. togoi in all experiments (p < 0.05). Reciprocal crosses (female An. paraliae x male An. lesteri) produced highly susceptible F1-hybrid progeny, with increased infection prevalence when compared to parental stocks (p < 0.05). Subsequently, the possibilities of introgression between high and low/moderate parasite susceptibility genes were investigated by cross-mating experiments (parental, reciprocal crosses, back crosses and repeated backcrosses). The results showed the possibility of introgression of B. malayi-susceptible genes between An. paraliae (low/moderate susceptibility) and An. lesteri (high susceptibility) based on increasing or decreasing susceptibility and normal larval development in the thoracic muscles of F3-hybrids. Additionally, melanization, an innate immune response with proven involvement in the susceptibility or refractoriness of mosquitoes to B. malayi parasites, was examined. Parasite degeneration and cell aggregation, and melanization were observed for first-stage larvae in the thoracic muscle fibers of hybrid mosquitoes. 2018-09-05T04:18:52Z 2018-09-05T04:18:52Z 2018-11-01 Journal 18736254 0001706X 2-s2.0-85050765688 10.1016/j.actatropica.2018.07.020 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050765688&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58000
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Immunology and Microbiology
Medicine
Veterinary
spellingShingle Agricultural and Biological Sciences
Immunology and Microbiology
Medicine
Veterinary
Watcharatip Dedkhad
Lyric C. Bartholomay
Bruce M. Christensen
Deepak Joshi
Kritsana Taai
Chayanit Hempolchom
Atiporn Saeung
Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi
description © 2018 Elsevier B.V. In Southeast Asia, Anopheles lesteri (recently synonymized with An. paraliae) is a competent vector for Plasmodium parasites, but its ability to transmit parasites that cause lymphatic filariasis has yet to be determined. In this study, the susceptibility of An. lesteri and An. paraliae to Brugia malayi parasites was determined by comparing with the control mosquito, Aedes togoi. We found that the infection prevalence per infected mosquito in An. paraliae was significantly lower than that in Ae. togoi in all experiments (p < 0.05). Reciprocal crosses (female An. paraliae x male An. lesteri) produced highly susceptible F1-hybrid progeny, with increased infection prevalence when compared to parental stocks (p < 0.05). Subsequently, the possibilities of introgression between high and low/moderate parasite susceptibility genes were investigated by cross-mating experiments (parental, reciprocal crosses, back crosses and repeated backcrosses). The results showed the possibility of introgression of B. malayi-susceptible genes between An. paraliae (low/moderate susceptibility) and An. lesteri (high susceptibility) based on increasing or decreasing susceptibility and normal larval development in the thoracic muscles of F3-hybrids. Additionally, melanization, an innate immune response with proven involvement in the susceptibility or refractoriness of mosquitoes to B. malayi parasites, was examined. Parasite degeneration and cell aggregation, and melanization were observed for first-stage larvae in the thoracic muscle fibers of hybrid mosquitoes.
format Journal
author Watcharatip Dedkhad
Lyric C. Bartholomay
Bruce M. Christensen
Deepak Joshi
Kritsana Taai
Chayanit Hempolchom
Atiporn Saeung
author_facet Watcharatip Dedkhad
Lyric C. Bartholomay
Bruce M. Christensen
Deepak Joshi
Kritsana Taai
Chayanit Hempolchom
Atiporn Saeung
author_sort Watcharatip Dedkhad
title Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi
title_short Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi
title_full Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi
title_fullStr Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi
title_full_unstemmed Effects of cross-mating on susceptibility of synonymous mosquitoes, Anopheles paraliae and Anopheles lesteri to infection with nocturnally subperiodic Brugia malayi
title_sort effects of cross-mating on susceptibility of synonymous mosquitoes, anopheles paraliae and anopheles lesteri to infection with nocturnally subperiodic brugia malayi
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050765688&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58000
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