Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods
Fifty-one human fecal specimens were collected from villagers inhabiting along Mae Kuang River, Ban Sob Tha, Pa Sang District, Lamphun Province, Thailand. By the formalin-ether sedimentation technique (FEST) under a light microscope, eggs of 3 helminth species, Haplorchis taichui, Ascaris lumbricoid...
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2014
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th-cmuir.6653943832-58192014-08-30T03:23:30Z Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods Wongsawad C. Wongsawad P. Chuboon S. Anuntalabhochai S. Fifty-one human fecal specimens were collected from villagers inhabiting along Mae Kuang River, Ban Sob Tha, Pa Sang District, Lamphun Province, Thailand. By the formalin-ether sedimentation technique (FEST) under a light microscope, eggs of 3 helminth species, Haplorchis taichui, Ascaris lumbricoides and unidentified hookworm species were detected with prevalences of 22,14 and 4%, respectively. PCR amplification with H. taichui specific primers showed that H. taichui specific amplicon 260 bp was generated in all FEST-positive specimens, and also in some FEST negative specimens. This H. taichui specific PCR method can be used to detect this parasite in all developmental stages and in both definitive and intermediate hosts, which should be useful in prevention and control programs. 2014-08-30T03:23:30Z 2014-08-30T03:23:30Z 2009 Article 01251562 19842375 http://www.scopus.com/inward/record.url?eid=2-s2.0-70349829696&partnerID=40&md5=26ecd4362707ba07eea45db43e30e6e7 http://www.ncbi.nlm.nih.gov/pubmed/19842375 http://cmuir.cmu.ac.th/handle/6653943832/5819 English |
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English |
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Fifty-one human fecal specimens were collected from villagers inhabiting along Mae Kuang River, Ban Sob Tha, Pa Sang District, Lamphun Province, Thailand. By the formalin-ether sedimentation technique (FEST) under a light microscope, eggs of 3 helminth species, Haplorchis taichui, Ascaris lumbricoides and unidentified hookworm species were detected with prevalences of 22,14 and 4%, respectively. PCR amplification with H. taichui specific primers showed that H. taichui specific amplicon 260 bp was generated in all FEST-positive specimens, and also in some FEST negative specimens. This H. taichui specific PCR method can be used to detect this parasite in all developmental stages and in both definitive and intermediate hosts, which should be useful in prevention and control programs. |
| format |
Article |
| author |
Wongsawad C. Wongsawad P. Chuboon S. Anuntalabhochai S. |
| spellingShingle |
Wongsawad C. Wongsawad P. Chuboon S. Anuntalabhochai S. Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods |
| author_facet |
Wongsawad C. Wongsawad P. Chuboon S. Anuntalabhochai S. |
| author_sort |
Wongsawad C. |
| title |
Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods |
| title_short |
Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods |
| title_full |
Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods |
| title_fullStr |
Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods |
| title_full_unstemmed |
Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods |
| title_sort |
copro-diagnosis of haplorchis taichui infection using sedimentation and pcr-based methods |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-70349829696&partnerID=40&md5=26ecd4362707ba07eea45db43e30e6e7 http://www.ncbi.nlm.nih.gov/pubmed/19842375 http://cmuir.cmu.ac.th/handle/6653943832/5819 |
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