Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk

© 2018, The Author(s). Rice husk (RH) is the major agricultural waste obtained during rice hulling process, which can be a sustainable source of xylooligosaccharide (XOS). The current study deals with the production of XOS from Thai rice husk using alkaline pretreatment and enzyme hydrolysis method....

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Main Authors: Nuntawat Khat-udomkiri, Bhagavathi Sundaram Sivamaruthi, Sasithorn Sirilun, Narissara Lailerd, Sartjin Peerajan, Chaiyavat Chaiyasut
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58199
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-581992018-09-05T04:33:52Z Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk Nuntawat Khat-udomkiri Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Narissara Lailerd Sartjin Peerajan Chaiyavat Chaiyasut Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine © 2018, The Author(s). Rice husk (RH) is the major agricultural waste obtained during rice hulling process, which can be a sustainable source of xylooligosaccharide (XOS). The current study deals with the production of XOS from Thai rice husk using alkaline pretreatment and enzyme hydrolysis method. The response surface methodology consisted of central composite design and Box–Behnken design was employed to achieve the maximum response in alkaline pretreatment and XOS production, respectively. The optimum conditions for alkaline pretreatment to recover maximum xylan yield were 12–18% of alkaline concentration, the temperature at 110–120 °C, and steaming time for 37.5–40 min. The FTIR results suggested that the extracted sample was the xylan fraction. The maximum XOS production of 17.35 ± 0.31 mg XOS per mL xylan was observed in the run conditions of 6.25 mg enzyme per g xylan, 9 h of incubation time, and 5% of xylan. The results revealed that the xylan extracted from RH by using an effective base couple with the steam application and the enzymatic hydrolysis help to maximize the yield of XOS, which can be further used in functional foods and dietary supplements. 2018-09-05T04:21:00Z 2018-09-05T04:21:00Z 2018-12-01 Journal 21910855 2-s2.0-85050151839 10.1186/s13568-018-0645-9 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050151839&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58199
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Nuntawat Khat-udomkiri
Bhagavathi Sundaram Sivamaruthi
Sasithorn Sirilun
Narissara Lailerd
Sartjin Peerajan
Chaiyavat Chaiyasut
Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk
description © 2018, The Author(s). Rice husk (RH) is the major agricultural waste obtained during rice hulling process, which can be a sustainable source of xylooligosaccharide (XOS). The current study deals with the production of XOS from Thai rice husk using alkaline pretreatment and enzyme hydrolysis method. The response surface methodology consisted of central composite design and Box–Behnken design was employed to achieve the maximum response in alkaline pretreatment and XOS production, respectively. The optimum conditions for alkaline pretreatment to recover maximum xylan yield were 12–18% of alkaline concentration, the temperature at 110–120 °C, and steaming time for 37.5–40 min. The FTIR results suggested that the extracted sample was the xylan fraction. The maximum XOS production of 17.35 ± 0.31 mg XOS per mL xylan was observed in the run conditions of 6.25 mg enzyme per g xylan, 9 h of incubation time, and 5% of xylan. The results revealed that the xylan extracted from RH by using an effective base couple with the steam application and the enzymatic hydrolysis help to maximize the yield of XOS, which can be further used in functional foods and dietary supplements.
format Journal
author Nuntawat Khat-udomkiri
Bhagavathi Sundaram Sivamaruthi
Sasithorn Sirilun
Narissara Lailerd
Sartjin Peerajan
Chaiyavat Chaiyasut
author_facet Nuntawat Khat-udomkiri
Bhagavathi Sundaram Sivamaruthi
Sasithorn Sirilun
Narissara Lailerd
Sartjin Peerajan
Chaiyavat Chaiyasut
author_sort Nuntawat Khat-udomkiri
title Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk
title_short Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk
title_full Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk
title_fullStr Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk
title_full_unstemmed Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk
title_sort optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050151839&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58199
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