Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. A hyphenated sequential injection ion chromatography–flow injection analysis (SIIC–FIA) system is proposed for post column derivatization capable of using murexide, an unstable but commonly available reagent, for the determination of cal...

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Main Authors: Somchai Lapanantnoppakhun, Lucksagoon Ganranoo, Supaporn Kradtap Hartwell, Kate Grudpan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58205
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-582052018-09-05T04:24:05Z Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture Somchai Lapanantnoppakhun Lucksagoon Ganranoo Supaporn Kradtap Hartwell Kate Grudpan Biochemistry, Genetics and Molecular Biology Chemistry © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. A hyphenated sequential injection ion chromatography–flow injection analysis (SIIC–FIA) system is proposed for post column derivatization capable of using murexide, an unstable but commonly available reagent, for the determination of calcium and magnesium in a mixture. A short ion exchange column was modified from a 4.6 mm × 10 mm C18 monolithic guard column. Calcium/magnesium mixture and murexide were used as model analytes and derivatizing agent, respectively. The FIA post column allows the use of unstable chromogenic reagents. The continuous flow of liquid also helped to stabilize the signal baseline during detection. A single concentration based calibration graph was demonstrated. The system requires low volumes of standard/sample (5–50 µL) and mobile phase (4.50 mL), and offers sample throughput at 8 h−1without the need for long column calibration. Detection limits were 0.5 and 0.1 µg for Ca2+and Mg2+, respectively. Working ranges were 1–5 µg Ca2+and 0.2–1 µg Mg2+. Analyses of well and drinking water samples were demonstrated and validated with the standard complexometric titration. 2018-09-05T04:21:04Z 2018-09-05T04:21:04Z 2018-09-01 Journal 16121112 00095893 2-s2.0-85050358067 10.1007/s10337-018-3575-4 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050358067&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58205
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Somchai Lapanantnoppakhun
Lucksagoon Ganranoo
Supaporn Kradtap Hartwell
Kate Grudpan
Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture
description © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. A hyphenated sequential injection ion chromatography–flow injection analysis (SIIC–FIA) system is proposed for post column derivatization capable of using murexide, an unstable but commonly available reagent, for the determination of calcium and magnesium in a mixture. A short ion exchange column was modified from a 4.6 mm × 10 mm C18 monolithic guard column. Calcium/magnesium mixture and murexide were used as model analytes and derivatizing agent, respectively. The FIA post column allows the use of unstable chromogenic reagents. The continuous flow of liquid also helped to stabilize the signal baseline during detection. A single concentration based calibration graph was demonstrated. The system requires low volumes of standard/sample (5–50 µL) and mobile phase (4.50 mL), and offers sample throughput at 8 h−1without the need for long column calibration. Detection limits were 0.5 and 0.1 µg for Ca2+and Mg2+, respectively. Working ranges were 1–5 µg Ca2+and 0.2–1 µg Mg2+. Analyses of well and drinking water samples were demonstrated and validated with the standard complexometric titration.
format Journal
author Somchai Lapanantnoppakhun
Lucksagoon Ganranoo
Supaporn Kradtap Hartwell
Kate Grudpan
author_facet Somchai Lapanantnoppakhun
Lucksagoon Ganranoo
Supaporn Kradtap Hartwell
Kate Grudpan
author_sort Somchai Lapanantnoppakhun
title Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture
title_short Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture
title_full Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture
title_fullStr Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture
title_full_unstemmed Sequential Injection Ion Chromatography with Flow Injection Post Column Derivatization Capable of Using the Unstable Reagent Murexide to Determine Calcium and Magnesium in a Mixture
title_sort sequential injection ion chromatography with flow injection post column derivatization capable of using the unstable reagent murexide to determine calcium and magnesium in a mixture
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050358067&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58205
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