The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice

© 2018, Chiang Mai University. All rights reserved. Rhizospheric actinomycetes were screened for bioactive compound production such as antibiotic, siderophore and cell wall degrading enzyme against Fusarium wilt disease and sheath blight disease in rice which are caused by Fusarium oxysporum and Rhi...

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Main Authors: Mathurot Chaiharn, Nikhom Sujada, Wasu Pathom-Aree, Saisamorn Lumyong
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58228
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spelling th-cmuir.6653943832-582282018-09-05T04:39:01Z The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice Mathurot Chaiharn Nikhom Sujada Wasu Pathom-Aree Saisamorn Lumyong Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2018, Chiang Mai University. All rights reserved. Rhizospheric actinomycetes were screened for bioactive compound production such as antibiotic, siderophore and cell wall degrading enzyme against Fusarium wilt disease and sheath blight disease in rice which are caused by Fusarium oxysporum and Rhizoctonia solani AG-2, respectively. A total of 150 strains was classified based on their cultural and physiological characterization such as color of aerial mycelium, color of substrate mycelium and spore chain morphology combined with cell wall composition analysis. They were classified as Streptomyces (52.7 %), non-Streptomyces (35.3 %) and unidentified (12 %). Involvement of cell wall degrading enzymes such as chitinase, cellulase and β-1,3-glucanase was investigated. Results showed that isolate RHI-43 produced high levels of chitinase (3.50 U/ml), cellulase (4.90 U/ml) and β-1,3-glucanase (0.45 U/ml) while isolate RHI-114 produced high levels of protease (15 U/ml). On the basis of dual culture assays, isolate RHI-39 and isolate RHI-146 were selected on the basis of broad spectrum activities and identified using cell wall composition analysis combined with 16S rDNA analysis and phylogenic classification. These isolates were identified as Streptomyces lydicus (for isolate RHI-39) and Streptomyces corchorussi (for isolate RHI-146). Further, culture filtrates of isolate RHI-146 inhibited the growth of all fungi tested which showed hyphal swelling and abnormal shapes of tested fungi under microscope. These results suggest that Streptomyces isolate RHI-39 and Streptomyces isolate RHI-146 may be utilized as an environmental friendly biocontrol agent against some important rice-pathogenic fungi in vitro. 2018-09-05T04:21:26Z 2018-09-05T04:21:26Z 2018-07-01 Journal 01252526 2-s2.0-85050296371 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050296371&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58228
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Mathurot Chaiharn
Nikhom Sujada
Wasu Pathom-Aree
Saisamorn Lumyong
The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice
description © 2018, Chiang Mai University. All rights reserved. Rhizospheric actinomycetes were screened for bioactive compound production such as antibiotic, siderophore and cell wall degrading enzyme against Fusarium wilt disease and sheath blight disease in rice which are caused by Fusarium oxysporum and Rhizoctonia solani AG-2, respectively. A total of 150 strains was classified based on their cultural and physiological characterization such as color of aerial mycelium, color of substrate mycelium and spore chain morphology combined with cell wall composition analysis. They were classified as Streptomyces (52.7 %), non-Streptomyces (35.3 %) and unidentified (12 %). Involvement of cell wall degrading enzymes such as chitinase, cellulase and β-1,3-glucanase was investigated. Results showed that isolate RHI-43 produced high levels of chitinase (3.50 U/ml), cellulase (4.90 U/ml) and β-1,3-glucanase (0.45 U/ml) while isolate RHI-114 produced high levels of protease (15 U/ml). On the basis of dual culture assays, isolate RHI-39 and isolate RHI-146 were selected on the basis of broad spectrum activities and identified using cell wall composition analysis combined with 16S rDNA analysis and phylogenic classification. These isolates were identified as Streptomyces lydicus (for isolate RHI-39) and Streptomyces corchorussi (for isolate RHI-146). Further, culture filtrates of isolate RHI-146 inhibited the growth of all fungi tested which showed hyphal swelling and abnormal shapes of tested fungi under microscope. These results suggest that Streptomyces isolate RHI-39 and Streptomyces isolate RHI-146 may be utilized as an environmental friendly biocontrol agent against some important rice-pathogenic fungi in vitro.
format Journal
author Mathurot Chaiharn
Nikhom Sujada
Wasu Pathom-Aree
Saisamorn Lumyong
author_facet Mathurot Chaiharn
Nikhom Sujada
Wasu Pathom-Aree
Saisamorn Lumyong
author_sort Mathurot Chaiharn
title The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice
title_short The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice
title_full The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice
title_fullStr The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice
title_full_unstemmed The antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice
title_sort antagonistic activity of bioactive compound producing streptomyces of fusarium wilt disease and sheath blight disease in rice
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050296371&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58228
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