Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae

© 2018, Chiang Mai University. All rights reserved. American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis ceran...

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Main Authors: Sasiprapa Krongdang, Jeffery S. Pettis, Geoffrey R. Williams, Panuwan Chantawannakul
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58260
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spelling th-cmuir.6653943832-582602018-09-05T04:39:15Z Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae Sasiprapa Krongdang Jeffery S. Pettis Geoffrey R. Williams Panuwan Chantawannakul Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2018, Chiang Mai University. All rights reserved. American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis cerana) to act antagonistically against P. larvae. Isolates were sampled from different locations on the fronts of A. cerana hives in Vietnam. A total of 69 isolates were obtained through a culture-dependent method and 16S rRNA gene sequencing showed affiliation to the phyla Firmicutes and Actinobacteria. Out of 69 isolates, 15 showed strong inhibitory activity against P. larvae; Bacillus pocheonensis (VN101) showed the largest zone of inhibition (26 ± 1 mm). In this study, the diversity and richness of antagonistic isolates indicated that Bacillus spp. are the most promising as inhibitors of P. larvae. These finding suggested that certain bacterial isolates can act as antagonist to control P. larvae and may have other biotechnological applications. 2018-09-05T04:21:45Z 2018-09-05T04:21:45Z 2018-05-01 Journal 01252526 2-s2.0-85049735962 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049735962&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58260
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Sasiprapa Krongdang
Jeffery S. Pettis
Geoffrey R. Williams
Panuwan Chantawannakul
Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae
description © 2018, Chiang Mai University. All rights reserved. American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis cerana) to act antagonistically against P. larvae. Isolates were sampled from different locations on the fronts of A. cerana hives in Vietnam. A total of 69 isolates were obtained through a culture-dependent method and 16S rRNA gene sequencing showed affiliation to the phyla Firmicutes and Actinobacteria. Out of 69 isolates, 15 showed strong inhibitory activity against P. larvae; Bacillus pocheonensis (VN101) showed the largest zone of inhibition (26 ± 1 mm). In this study, the diversity and richness of antagonistic isolates indicated that Bacillus spp. are the most promising as inhibitors of P. larvae. These finding suggested that certain bacterial isolates can act as antagonist to control P. larvae and may have other biotechnological applications.
format Journal
author Sasiprapa Krongdang
Jeffery S. Pettis
Geoffrey R. Williams
Panuwan Chantawannakul
author_facet Sasiprapa Krongdang
Jeffery S. Pettis
Geoffrey R. Williams
Panuwan Chantawannakul
author_sort Sasiprapa Krongdang
title Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae
title_short Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae
title_full Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae
title_fullStr Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae
title_full_unstemmed Screening of antagonistic bacterial isolates from hives of Apis cerana in Vietnam against the causal agent of American foulbrood of Honey Bees, Paenibacillus larvae
title_sort screening of antagonistic bacterial isolates from hives of apis cerana in vietnam against the causal agent of american foulbrood of honey bees, paenibacillus larvae
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049735962&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58260
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