Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in...

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Main Authors: Ning Zhang, Yuxuan Fan, Chen Li, Qiming Wang, Noppol Leksawasdi, Fuli Li, Shian Wang
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58263
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-582632018-09-05T04:30:31Z Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts Ning Zhang Yuxuan Fan Chen Li Qiming Wang Noppol Leksawasdi Fuli Li Shian Wang Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether the classical nuclear DNA staining method for ascomycetous yeasts is applicable to basidiomycetous yeasts. In this study, 5 yeasts ineffectively stained by the classical propidium iodide (PI) staining method were identified from 23 representative basidiomycetous yeasts. Pretreatment of cells using dimethyl sulfoxide (DMSO) or snailase markedly improved cell penetration to PI and thus enabled DNA content determination by flow cytometry on the recalcitrant yeasts. The pretreatments are efficient, simple, and fast, avoiding tedious mutagenesis or genetic engineering used in previous reports. The heterogeneity of cell penetration to PI among basidiomycetous yeasts was attributed to the discrepancy in cell wall polysaccharides instead of capsule or plasma membrane. This study also indicated that care must be taken in attributing PI-negative staining as viable cells when studying non-model microorganisms. 2018-09-05T04:21:47Z 2018-09-05T04:21:47Z 2018-05-01 Journal 14320614 01757598 2-s2.0-85044392159 10.1007/s00253-018-8906-8 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85044392159&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58263
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Ning Zhang
Yuxuan Fan
Chen Li
Qiming Wang
Noppol Leksawasdi
Fuli Li
Shian Wang
Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
description © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether the classical nuclear DNA staining method for ascomycetous yeasts is applicable to basidiomycetous yeasts. In this study, 5 yeasts ineffectively stained by the classical propidium iodide (PI) staining method were identified from 23 representative basidiomycetous yeasts. Pretreatment of cells using dimethyl sulfoxide (DMSO) or snailase markedly improved cell penetration to PI and thus enabled DNA content determination by flow cytometry on the recalcitrant yeasts. The pretreatments are efficient, simple, and fast, avoiding tedious mutagenesis or genetic engineering used in previous reports. The heterogeneity of cell penetration to PI among basidiomycetous yeasts was attributed to the discrepancy in cell wall polysaccharides instead of capsule or plasma membrane. This study also indicated that care must be taken in attributing PI-negative staining as viable cells when studying non-model microorganisms.
format Journal
author Ning Zhang
Yuxuan Fan
Chen Li
Qiming Wang
Noppol Leksawasdi
Fuli Li
Shian Wang
author_facet Ning Zhang
Yuxuan Fan
Chen Li
Qiming Wang
Noppol Leksawasdi
Fuli Li
Shian Wang
author_sort Ning Zhang
title Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
title_short Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
title_full Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
title_fullStr Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
title_full_unstemmed Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
title_sort cell permeability and nuclear dna staining by propidium iodide in basidiomycetous yeasts
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85044392159&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58263
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