Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in...
Saved in:
Main Authors: | , , , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85044392159&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58263 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-58263 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-582632018-09-05T04:30:31Z Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts Ning Zhang Yuxuan Fan Chen Li Qiming Wang Noppol Leksawasdi Fuli Li Shian Wang Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether the classical nuclear DNA staining method for ascomycetous yeasts is applicable to basidiomycetous yeasts. In this study, 5 yeasts ineffectively stained by the classical propidium iodide (PI) staining method were identified from 23 representative basidiomycetous yeasts. Pretreatment of cells using dimethyl sulfoxide (DMSO) or snailase markedly improved cell penetration to PI and thus enabled DNA content determination by flow cytometry on the recalcitrant yeasts. The pretreatments are efficient, simple, and fast, avoiding tedious mutagenesis or genetic engineering used in previous reports. The heterogeneity of cell penetration to PI among basidiomycetous yeasts was attributed to the discrepancy in cell wall polysaccharides instead of capsule or plasma membrane. This study also indicated that care must be taken in attributing PI-negative staining as viable cells when studying non-model microorganisms. 2018-09-05T04:21:47Z 2018-09-05T04:21:47Z 2018-05-01 Journal 14320614 01757598 2-s2.0-85044392159 10.1007/s00253-018-8906-8 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85044392159&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58263 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
topic |
Biochemistry, Genetics and Molecular Biology Immunology and Microbiology |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Ning Zhang Yuxuan Fan Chen Li Qiming Wang Noppol Leksawasdi Fuli Li Shian Wang Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts |
description |
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether the classical nuclear DNA staining method for ascomycetous yeasts is applicable to basidiomycetous yeasts. In this study, 5 yeasts ineffectively stained by the classical propidium iodide (PI) staining method were identified from 23 representative basidiomycetous yeasts. Pretreatment of cells using dimethyl sulfoxide (DMSO) or snailase markedly improved cell penetration to PI and thus enabled DNA content determination by flow cytometry on the recalcitrant yeasts. The pretreatments are efficient, simple, and fast, avoiding tedious mutagenesis or genetic engineering used in previous reports. The heterogeneity of cell penetration to PI among basidiomycetous yeasts was attributed to the discrepancy in cell wall polysaccharides instead of capsule or plasma membrane. This study also indicated that care must be taken in attributing PI-negative staining as viable cells when studying non-model microorganisms. |
format |
Journal |
author |
Ning Zhang Yuxuan Fan Chen Li Qiming Wang Noppol Leksawasdi Fuli Li Shian Wang |
author_facet |
Ning Zhang Yuxuan Fan Chen Li Qiming Wang Noppol Leksawasdi Fuli Li Shian Wang |
author_sort |
Ning Zhang |
title |
Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts |
title_short |
Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts |
title_full |
Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts |
title_fullStr |
Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts |
title_full_unstemmed |
Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts |
title_sort |
cell permeability and nuclear dna staining by propidium iodide in basidiomycetous yeasts |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85044392159&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58263 |
_version_ |
1681425032705736704 |