Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics

© 2018 Elsevier Inc. The incidence of cardiovascular disease and metabolic syndrome increases after the onset of menopause, suggesting estrogen has a vital role in their prevention. Mitochondrial dynamics are known to play an important role in the maintenance of cardiac physiological function. Howev...

Full description

Saved in:
Bibliographic Details
Main Authors: Wanitchaya Minta, Siripong Palee, Duangkamol Mantor, Wissuta Sutham, Thidarat Jaiwongkam, Sasiwan Kerdphoo, Wasana Pratchayasakul, Sirinart Kumfu, Siriporn C. Chattipakorn, Nipon Chattipakorn
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041171644&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58281
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-58281
record_format dspace
spelling th-cmuir.6653943832-582812018-09-05T04:22:07Z Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics Wanitchaya Minta Siripong Palee Duangkamol Mantor Wissuta Sutham Thidarat Jaiwongkam Sasiwan Kerdphoo Wasana Pratchayasakul Sirinart Kumfu Siriporn C. Chattipakorn Nipon Chattipakorn Biochemistry, Genetics and Molecular Biology © 2018 Elsevier Inc. The incidence of cardiovascular disease and metabolic syndrome increases after the onset of menopause, suggesting estrogen has a vital role in their prevention. Mitochondrial dynamics are known to play an important role in the maintenance of cardiac physiological function. However, the effects of estrogen deprivation on cardiometabolic status and cardiac mitochondrial dynamics under conditions of obese-insulin resistance have never been investigated. We hypothesized that estrogen deprivation aggravates cardiac dysfunction through increased cardiac mitochondrial fission in obese-insulin resistant rats. Female rats were fed on either a high fat (HFD, 57.60% fat) or normal (ND, 19.77% fat) diet for 13 weeks. The rats were then divided into 4 groups. Two sham groups (HFS and NDS) and 2 operated or ovariectomized (HFO and NDO) groups (n = 8/group). Six weeks after surgery, metabolic status, heart rate variability (HRV), left ventricular (LV) function, cardiac mitochondrial function and dynamics, and metabolic parameters were determined. Insulin resistance developed in NDO, HFS and HFO rats as indicated by increased plasma insulin and HOMA index. Although rats in both NDO and HFS groups had markedly impaired LV function indicated by reduced %LVFS and impaired cardiac mitochondrial function, rats in the HFO group had the most severe impairments. Moreover, the estrogen deprived rats (NDO and HFO) had increased cardiac mitochondrial fission through activation of phosphorylation of Drp-1 at serine 616. Our findings indicated that estrogen deprivation caused the worsening of LV dysfunction through increased cardiac mitochondrial fission in obese-insulin resistant rats. 2018-09-05T04:22:07Z 2018-09-05T04:22:07Z 2018-03-01 Journal 18736815 05315565 2-s2.0-85041171644 10.1016/j.exger.2018.01.006 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041171644&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58281
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Wanitchaya Minta
Siripong Palee
Duangkamol Mantor
Wissuta Sutham
Thidarat Jaiwongkam
Sasiwan Kerdphoo
Wasana Pratchayasakul
Sirinart Kumfu
Siriporn C. Chattipakorn
Nipon Chattipakorn
Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics
description © 2018 Elsevier Inc. The incidence of cardiovascular disease and metabolic syndrome increases after the onset of menopause, suggesting estrogen has a vital role in their prevention. Mitochondrial dynamics are known to play an important role in the maintenance of cardiac physiological function. However, the effects of estrogen deprivation on cardiometabolic status and cardiac mitochondrial dynamics under conditions of obese-insulin resistance have never been investigated. We hypothesized that estrogen deprivation aggravates cardiac dysfunction through increased cardiac mitochondrial fission in obese-insulin resistant rats. Female rats were fed on either a high fat (HFD, 57.60% fat) or normal (ND, 19.77% fat) diet for 13 weeks. The rats were then divided into 4 groups. Two sham groups (HFS and NDS) and 2 operated or ovariectomized (HFO and NDO) groups (n = 8/group). Six weeks after surgery, metabolic status, heart rate variability (HRV), left ventricular (LV) function, cardiac mitochondrial function and dynamics, and metabolic parameters were determined. Insulin resistance developed in NDO, HFS and HFO rats as indicated by increased plasma insulin and HOMA index. Although rats in both NDO and HFS groups had markedly impaired LV function indicated by reduced %LVFS and impaired cardiac mitochondrial function, rats in the HFO group had the most severe impairments. Moreover, the estrogen deprived rats (NDO and HFO) had increased cardiac mitochondrial fission through activation of phosphorylation of Drp-1 at serine 616. Our findings indicated that estrogen deprivation caused the worsening of LV dysfunction through increased cardiac mitochondrial fission in obese-insulin resistant rats.
format Journal
author Wanitchaya Minta
Siripong Palee
Duangkamol Mantor
Wissuta Sutham
Thidarat Jaiwongkam
Sasiwan Kerdphoo
Wasana Pratchayasakul
Sirinart Kumfu
Siriporn C. Chattipakorn
Nipon Chattipakorn
author_facet Wanitchaya Minta
Siripong Palee
Duangkamol Mantor
Wissuta Sutham
Thidarat Jaiwongkam
Sasiwan Kerdphoo
Wasana Pratchayasakul
Sirinart Kumfu
Siriporn C. Chattipakorn
Nipon Chattipakorn
author_sort Wanitchaya Minta
title Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics
title_short Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics
title_full Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics
title_fullStr Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics
title_full_unstemmed Estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics
title_sort estrogen deprivation aggravates cardiometabolic dysfunction in obese-insulin resistant rats through the impairment of cardiac mitochondrial dynamics
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041171644&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58281
_version_ 1681425036003508224