O-GlcNAcylation in oral squamous cell carcinoma

© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background: Two post-translational mechanisms commonly demonstrated in various cancers are protein phosphorylation and glycosylation by O-linked β-N-acetylglucosamine (O-GlcNAc). However, only phosphorylation of the epiderm...

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Main Authors: Tassaporn Kongkaew, Win Pa Pa Aung, Chayarop Supanchart, Anupong Makeudom, Sarawat Langsa-ard, Thanapat Sastraruji, Ponlatham Chaiyarit, Suttichai Krisanaprakornkit
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58288
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spelling th-cmuir.6653943832-582882018-09-05T04:35:52Z O-GlcNAcylation in oral squamous cell carcinoma Tassaporn Kongkaew Win Pa Pa Aung Chayarop Supanchart Anupong Makeudom Sarawat Langsa-ard Thanapat Sastraruji Ponlatham Chaiyarit Suttichai Krisanaprakornkit Biochemistry, Genetics and Molecular Biology Dentistry Medicine © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background: Two post-translational mechanisms commonly demonstrated in various cancers are protein phosphorylation and glycosylation by O-linked β-N-acetylglucosamine (O-GlcNAc). However, only phosphorylation of the epidermal growth factor receptor (EGFR)/Akt pathway has been reported in oral squamous cell carcinoma (OSCC). Therefore, we aimed to determine both post-translational modifications in OSCC tissues and in oral cancer cells compared to normal tissues and oral keratinocytes and to find correlations of these modifications with histological grading. Methods: Thirty-two OSCC and ten normal formalin-fixed and paraffin-embedded sections were probed with the anti-O-GlcNAc, anti-O-GlcNAc transferase (OGT), anti-phosphorylated-EGFRtyr1173, and anti-phosphorylated-Aktser473antibodies following standard immunohistochemistry. The immunohistochemical (IHC) score was determined using the Fromowitz standard. Whole cell lysates of oral cancer cells and normal oral keratinocytes were immunoblotted with the anti-O-GlcNAc antibody. Results: The median IHC scores of O-GlcNAc or OGT between OSCC and normal tissues were not different, whereas those of phosphorylated-EGFRtyr1173and phosphorylated-Aktser473were significantly higher in OSCC than normal tissues (P <.001 and P <.01, respectively). Similarly, expression of O-GlcNAcylated proteins in oral cancer cells and normal oral keratinocytes did not differ. In the OSCC group, the median IHC scores of O-GlcNAc and OGT were significantly lower than those of phosphorylated-EGFRtyr1173and phosphorylated-Aktser473(P <.01 and P <.001, respectively). The IHC scores of O-GlcNAc or OGT were not determined to correlate with histological grading. Conclusion: Unlike other types of cancers, our findings demonstrate that the levels of O-GlcNAcylation are not significantly increased in OSCC tissues or in oral cancer cells and are not associated with the histological grading of OSCC. 2018-09-05T04:22:11Z 2018-09-05T04:22:11Z 2018-03-01 Journal 16000714 09042512 2-s2.0-85041137618 10.1111/jop.12680 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041137618&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58288
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Dentistry
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Dentistry
Medicine
Tassaporn Kongkaew
Win Pa Pa Aung
Chayarop Supanchart
Anupong Makeudom
Sarawat Langsa-ard
Thanapat Sastraruji
Ponlatham Chaiyarit
Suttichai Krisanaprakornkit
O-GlcNAcylation in oral squamous cell carcinoma
description © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background: Two post-translational mechanisms commonly demonstrated in various cancers are protein phosphorylation and glycosylation by O-linked β-N-acetylglucosamine (O-GlcNAc). However, only phosphorylation of the epidermal growth factor receptor (EGFR)/Akt pathway has been reported in oral squamous cell carcinoma (OSCC). Therefore, we aimed to determine both post-translational modifications in OSCC tissues and in oral cancer cells compared to normal tissues and oral keratinocytes and to find correlations of these modifications with histological grading. Methods: Thirty-two OSCC and ten normal formalin-fixed and paraffin-embedded sections were probed with the anti-O-GlcNAc, anti-O-GlcNAc transferase (OGT), anti-phosphorylated-EGFRtyr1173, and anti-phosphorylated-Aktser473antibodies following standard immunohistochemistry. The immunohistochemical (IHC) score was determined using the Fromowitz standard. Whole cell lysates of oral cancer cells and normal oral keratinocytes were immunoblotted with the anti-O-GlcNAc antibody. Results: The median IHC scores of O-GlcNAc or OGT between OSCC and normal tissues were not different, whereas those of phosphorylated-EGFRtyr1173and phosphorylated-Aktser473were significantly higher in OSCC than normal tissues (P <.001 and P <.01, respectively). Similarly, expression of O-GlcNAcylated proteins in oral cancer cells and normal oral keratinocytes did not differ. In the OSCC group, the median IHC scores of O-GlcNAc and OGT were significantly lower than those of phosphorylated-EGFRtyr1173and phosphorylated-Aktser473(P <.01 and P <.001, respectively). The IHC scores of O-GlcNAc or OGT were not determined to correlate with histological grading. Conclusion: Unlike other types of cancers, our findings demonstrate that the levels of O-GlcNAcylation are not significantly increased in OSCC tissues or in oral cancer cells and are not associated with the histological grading of OSCC.
format Journal
author Tassaporn Kongkaew
Win Pa Pa Aung
Chayarop Supanchart
Anupong Makeudom
Sarawat Langsa-ard
Thanapat Sastraruji
Ponlatham Chaiyarit
Suttichai Krisanaprakornkit
author_facet Tassaporn Kongkaew
Win Pa Pa Aung
Chayarop Supanchart
Anupong Makeudom
Sarawat Langsa-ard
Thanapat Sastraruji
Ponlatham Chaiyarit
Suttichai Krisanaprakornkit
author_sort Tassaporn Kongkaew
title O-GlcNAcylation in oral squamous cell carcinoma
title_short O-GlcNAcylation in oral squamous cell carcinoma
title_full O-GlcNAcylation in oral squamous cell carcinoma
title_fullStr O-GlcNAcylation in oral squamous cell carcinoma
title_full_unstemmed O-GlcNAcylation in oral squamous cell carcinoma
title_sort o-glcnacylation in oral squamous cell carcinoma
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041137618&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58288
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