Evaluation of the stability of propolis granule and toxicity study in wistar rats

© 2018, Chiang Mai University. All rights reserved. Nowadays, incorporation of natural products into conventional medicine has become a promising approach to improve the health benefits of conventional medicine. Propolis is composed of several chemical constituents which have various pharmacological...

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Main Authors: Supakit Khacha-Ananda, Kanokporn Saenphet, Supap Saenphet, Khajornsak Tragoolpua, Panuwan Chantawannakul, Yingmanee Tragoolpua
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58322
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spelling th-cmuir.6653943832-583222018-09-05T04:39:41Z Evaluation of the stability of propolis granule and toxicity study in wistar rats Supakit Khacha-Ananda Kanokporn Saenphet Supap Saenphet Khajornsak Tragoolpua Panuwan Chantawannakul Yingmanee Tragoolpua Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2018, Chiang Mai University. All rights reserved. Nowadays, incorporation of natural products into conventional medicine has become a promising approach to improve the health benefits of conventional medicine. Propolis is composed of several chemical constituents which have various pharmacological activities and which are widely used in apitherapy. However, evidence regarding toxicity of propolis products is required. Therefore, stability of propolis granule, as well as its acute and subchronic toxicity on Wistar rats, was investigated. The propolis granule was produced from propolis extract sourced from the Nan province, Thailand. The stability of propolis granule was determined from the total phenolic content and the total flavonoid content of propolis granule. The total phenolic content and the total flavonoid content of propolis granule which was kept at 4°C and the propolis granule which was kept at room temperature were not significantly different throughout 1 year. With regard to acute toxicity, we found that the behavior and physiological changes of the Wistar rats were normal after giving them propolis granule. After propolis granule was orally given to the rats for 60 days, there was no obvious sign of the propolis granule inducing any toxicity throughout the period of experiment. Significant increase in the mean corpuscular volume and decrease in the blood urea nitrogen, creatinine, and aspartate aminotransferase were observed in the rats. Likewise, histopathology examinations of the liver and the kidney did not reveal any morphological alteration. In light of these findings, we concluded that propolis granule does not induce any acute or subchronic toxicity in rats. This information can be further applied with regard to preparation of supplementary diet from the propolis extract for consumption. 2018-09-05T04:22:39Z 2018-09-05T04:22:39Z 2018-01-01 Journal 01252526 2-s2.0-85040917694 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85040917694&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58322
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Supakit Khacha-Ananda
Kanokporn Saenphet
Supap Saenphet
Khajornsak Tragoolpua
Panuwan Chantawannakul
Yingmanee Tragoolpua
Evaluation of the stability of propolis granule and toxicity study in wistar rats
description © 2018, Chiang Mai University. All rights reserved. Nowadays, incorporation of natural products into conventional medicine has become a promising approach to improve the health benefits of conventional medicine. Propolis is composed of several chemical constituents which have various pharmacological activities and which are widely used in apitherapy. However, evidence regarding toxicity of propolis products is required. Therefore, stability of propolis granule, as well as its acute and subchronic toxicity on Wistar rats, was investigated. The propolis granule was produced from propolis extract sourced from the Nan province, Thailand. The stability of propolis granule was determined from the total phenolic content and the total flavonoid content of propolis granule. The total phenolic content and the total flavonoid content of propolis granule which was kept at 4°C and the propolis granule which was kept at room temperature were not significantly different throughout 1 year. With regard to acute toxicity, we found that the behavior and physiological changes of the Wistar rats were normal after giving them propolis granule. After propolis granule was orally given to the rats for 60 days, there was no obvious sign of the propolis granule inducing any toxicity throughout the period of experiment. Significant increase in the mean corpuscular volume and decrease in the blood urea nitrogen, creatinine, and aspartate aminotransferase were observed in the rats. Likewise, histopathology examinations of the liver and the kidney did not reveal any morphological alteration. In light of these findings, we concluded that propolis granule does not induce any acute or subchronic toxicity in rats. This information can be further applied with regard to preparation of supplementary diet from the propolis extract for consumption.
format Journal
author Supakit Khacha-Ananda
Kanokporn Saenphet
Supap Saenphet
Khajornsak Tragoolpua
Panuwan Chantawannakul
Yingmanee Tragoolpua
author_facet Supakit Khacha-Ananda
Kanokporn Saenphet
Supap Saenphet
Khajornsak Tragoolpua
Panuwan Chantawannakul
Yingmanee Tragoolpua
author_sort Supakit Khacha-Ananda
title Evaluation of the stability of propolis granule and toxicity study in wistar rats
title_short Evaluation of the stability of propolis granule and toxicity study in wistar rats
title_full Evaluation of the stability of propolis granule and toxicity study in wistar rats
title_fullStr Evaluation of the stability of propolis granule and toxicity study in wistar rats
title_full_unstemmed Evaluation of the stability of propolis granule and toxicity study in wistar rats
title_sort evaluation of the stability of propolis granule and toxicity study in wistar rats
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85040917694&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58322
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