Evaluation of immunochromatographic test for dual detection of noroviruses and group A rotaviruses in stool samples

• Main Authors: Pattara Khamrin, Kattareeya Kumthip, Aksara Thongprachum, Sayaka Takanashi, Shoko Okitsu, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048899866&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/58334
• Institution: Chiang Mai University

© 2018 Verlag Klinisches Labor GmbH. All rights reserved. Background: Viral enteric infections, such as noroviruses and rotaviruses are considered as the major causes of acute gastroenteritis in young children and elderly people all over the world. These viruses are responsible for numerous outbreaks of nonbacterial gastroenteritis in several settings such as hospitals, day care centers, nursing homes, and restaurants. Therefore, a rapid and sensitive diagnostic tool for virus detections could be helpful for disease control. Methods: A new immunochromatographic test for the dual detection of noroviruses and group A rotaviruses was evaluated by using specimens that were known to be positive for noroviruses and rotaviruses, as well as other diarrheal viruses. Results: The sensitivity of detections for noroviruses and group A rotaviruses were 89.5% and 100%, respectively, while the specificity was 100% for both viruses. This immunochromatographic test was reactive to several norovirus and group A rotavirus genotypes (NoV GII.2, GII.3, GII.4, GII.6, GII.7, GII.17 and RVA G1P[8], G2P[4], G3P[8], G8P[8], G9P[8]). Conclusions: The speed and ease of use of the immunochromatographic test makes this kit particularly attractive as an alternative method for the detections of noroviruses and group A rotaviruses in the primary care unit.