A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline soluti...
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th-cmuir.6653943832-58782014-08-30T03:23:34Z A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait Khonyoung S. Kradtap Hartwell S. Jakmunee J. Lapanantnoppakhun S. Sanguansermsri T. Grudpan K. Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume. 2009 © The Japan Society for Analytical Chemistry. 2014-08-30T03:23:34Z 2014-08-30T03:23:34Z 2009 Article 09106340 10.2116/analsci.25.819 19531894 ANSCE http://www.scopus.com/inward/record.url?eid=2-s2.0-68249089807&partnerID=40&md5=27a27aaa887591994b933d19034b261e http://www.ncbi.nlm.nih.gov/pubmed/19531894 http://cmuir.cmu.ac.th/handle/6653943832/5878 English |
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Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume. 2009 © The Japan Society for Analytical Chemistry. |
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Khonyoung S. Kradtap Hartwell S. Jakmunee J. Lapanantnoppakhun S. Sanguansermsri T. Grudpan K. |
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Khonyoung S. Kradtap Hartwell S. Jakmunee J. Lapanantnoppakhun S. Sanguansermsri T. Grudpan K. A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait |
author_facet |
Khonyoung S. Kradtap Hartwell S. Jakmunee J. Lapanantnoppakhun S. Sanguansermsri T. Grudpan K. |
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Khonyoung S. |
title |
A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait |
title_short |
A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait |
title_full |
A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait |
title_fullStr |
A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait |
title_full_unstemmed |
A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait |
title_sort |
stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: possibility for automatic screening of beta-thalassemia trait |
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2014 |
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http://www.scopus.com/inward/record.url?eid=2-s2.0-68249089807&partnerID=40&md5=27a27aaa887591994b933d19034b261e http://www.ncbi.nlm.nih.gov/pubmed/19531894 http://cmuir.cmu.ac.th/handle/6653943832/5878 |
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