A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait

Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline soluti...

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Main Authors: Khonyoung S., Kradtap Hartwell S., Jakmunee J., Lapanantnoppakhun S., Sanguansermsri T., Grudpan K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-68249089807&partnerID=40&md5=27a27aaa887591994b933d19034b261e
http://www.ncbi.nlm.nih.gov/pubmed/19531894
http://cmuir.cmu.ac.th/handle/6653943832/5878
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-58782014-08-30T03:23:34Z A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait Khonyoung S. Kradtap Hartwell S. Jakmunee J. Lapanantnoppakhun S. Sanguansermsri T. Grudpan K. Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume. 2009 © The Japan Society for Analytical Chemistry. 2014-08-30T03:23:34Z 2014-08-30T03:23:34Z 2009 Article 09106340 10.2116/analsci.25.819 19531894 ANSCE http://www.scopus.com/inward/record.url?eid=2-s2.0-68249089807&partnerID=40&md5=27a27aaa887591994b933d19034b261e http://www.ncbi.nlm.nih.gov/pubmed/19531894 http://cmuir.cmu.ac.th/handle/6653943832/5878 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume. 2009 © The Japan Society for Analytical Chemistry.
format Article
author Khonyoung S.
Kradtap Hartwell S.
Jakmunee J.
Lapanantnoppakhun S.
Sanguansermsri T.
Grudpan K.
spellingShingle Khonyoung S.
Kradtap Hartwell S.
Jakmunee J.
Lapanantnoppakhun S.
Sanguansermsri T.
Grudpan K.
A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
author_facet Khonyoung S.
Kradtap Hartwell S.
Jakmunee J.
Lapanantnoppakhun S.
Sanguansermsri T.
Grudpan K.
author_sort Khonyoung S.
title A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_short A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_full A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_fullStr A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_full_unstemmed A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_sort stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: possibility for automatic screening of beta-thalassemia trait
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-68249089807&partnerID=40&md5=27a27aaa887591994b933d19034b261e
http://www.ncbi.nlm.nih.gov/pubmed/19531894
http://cmuir.cmu.ac.th/handle/6653943832/5878
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