Role of chitin binding domain of chitinase A of streptomyces cyaneus SP-27 in protoplast formation from schizophyllum commune

• Main Authors: Yano S., Honda A., Rattanakit-Chandet N., Yuta N., Wakayama M., Plikomol A., Tachiki T.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-65349158231&partnerID=40&md5=58dd1e537d8df3f93b1a0fc60622ee67; http://cmuir.cmu.ac.th/handle/6653943832/5904
• Institution: Chiang Mai University

Chitinase A (CHIA) of Streptomyces cyaneus SP-27 forms protoplasts from Schizophyllum commune mycelia when it is combined with α-1,3-glucanase of Bacillus circulans KA-304. An N-terminal chitin-binding domain truncated mutant (CatCHIA), which was expressed in Escherichia coli Rosetta-gami B (DE 3), lost most of its colloidal chitin- and powder chitin-binding activity. The colloidal chitin-hydrolyzing, the powder chitinhydrolyzing, and the protoplast-forming activities of CatCHIA were lower than those of CHIA, suggesting that the chitin-binding domain contributes to the hydrolysis of chitin in the cell-wall of S. commune.